| Field | Specification |
|---|---|
| Organism | |
| Applications | |
| Assay type | |
| Assay target | |
| Assay area | |
| Target type | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Drug Target Assay Kit
T4 DNA ligase is the DNA ligase of bacteriophage T4.
Kit vs Kit Plus
The only difference is the enzyme: T4L100K does not include ligase, while T4L100KE also includes 44 µl of 100 x T4 DNA ligase (5000 U/ml). Both are for 100 assays.
| Kit (enzyme not included), 100 assays (T4L100K) | 100 assays |
|---|---|
| Kit Plus (enzyme included), 100 assays (T4L100KE) | 100 assays |
Kit Components
| Component | Kit (enzyme not included), 100 assays (T4L100K) | Kit Plus (enzyme included), 100 assays (T4L100KE) |
|---|---|---|
| 10 x Buffer | 500 µl | 500 µl |
| 100 x DNA substrate | 44 µl | 44 µl |
| 100 x ATP (2 mM) | 44 µl | 44 µl |
| Reagent T | 21 ml | 21 ml |
| 10 x Dye | 220 µl | 220 µl |
| 100 x T4 DNA ligase (5000 U/ml) | Not included | 44 µl |
Specifications
| Assay Target | T4 DNA ligase activity |
|---|---|
| Target Type | DNA Ligase |
| Organism | Bacteriophage T4 |
| Assay Type | Enzyme Activity Assay; Inhibitor Screening Assay |
| Size | 100 assays |
| Storage | Please contact us to confirm storage conditions. |
| Shipping | Shipped frozen. |
Protocol
A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Safety & Handling
For Research Use Only (RUO). Not for use in diagnostic or therapeutic procedures. The Bacteriophage T4 DNA Ligase Assay Kit is shipped frozen. Follow standard laboratory safety practices when handling the reagents. Please contact us to confirm storage conditions.
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Q.What does this kit measure?
A.It measures the activity of bacteriophage T4 DNA ligase on the supplied DNA substrate in the presence of ATP, with Reagent T and a dye used for the readout.
Q.What is the difference between the options?
A.The only difference is the enzyme: T4L100K does not include ligase, while T4L100KE also includes 44 µl of 100 x T4 DNA ligase (5000 U/ml). Both are for 100 assays.
Q.Is a protocol available?
A.A detailed protocol is not available online for this kit. Please contact us and we will request the instructions from the manufacturer for you.
Q.How should the kit be stored?
A.The Bacteriophage T4 DNA Ligase Assay Kit is shipped frozen. Please contact us for long-term storage conditions.
Can’t find the exact format you need? We can help you source or customize this T4 DNA ligase activity assay — including larger pack sizes, bulk reagents, bulk enzyme, or a protocol adapted to your samples. Click Talk to a Scientist, email support@biohippo.com, or explore our Research Services. Our team will be in touch shortly.
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