| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Basic Fibroblast Growth Factor , FGF2, bFGF |
| Biological Activity | |
| Endotoxin Level | |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Recombinant human soluble basic FGF (GenBank Accession No. NM_002006) is a 17.3 kDa (134 aa-288 aa) protein expressed in an E. coli expression system.
Product Description
E. coli ExpressionRecombinant Basic FGF Human protein is produced using a validated E. coli expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | P09038 |
|---|---|
| Expression System | E. coli |
| Amino Acids / Region | 134 - 288 |
| Molecular Weight | 17.3 kDa |
| Purity | ≥95% by SDS-PAGE and HPLC analysis. |
| Endotoxin | <1 EU/µg (bFGF, by LAL method) |
| Formulation | Lyophilized from a 0.2 µm filtered solution containing 1M NaCl and 20 mM Phospha… |
| Storage | ≥2 years (lyophilized) at -20°C. |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles.
This protein was produced in E. coli. Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 134 - 288. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
≥95% by SDS-PAGE and HPLC analysis. as determined by SDS-PAGE. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful for cell culture and for the study of signaling pathways. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
≥2 years (lyophilized) at -20°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Matsui, Y., et al. Cell 70(5), 841-847 (1992).
- Segev, H, et al. Stem Cells. 22(3), 265-74 (2002).
