| Field | Specification |
|---|---|
| Target | |
| Alternative names | Antigen identified by monoclonal F8 ; AU; Auberger B antigen; B CAM cell surface glycoprotein; B cell adhesion molecule; B-CAM cell surface glycoprotein; Basal cell adhesion molecule (Lu and Au blood groups); Basal cell adhesion molecule (Lutheran blood group); Basal cell adhesion molecule; Basal cell adhesion molecule Lu and Au blood groups; Basal cell adhesion molecule Lutheran blood group; Bcam; BCAM_HUMAN; CD239; CD239 antigen; F8/G253 antigen; Glycoprotein 95kDa; LU; Lutheran; Lutheran antigen; Lutheran blood group (Auberger b antigen included); Lutheran blood group Auberger b antigen included ; Lutheran blood group glycoprotein; MSK19 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human Basal cell adhesion molecule protein (447-547AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-BCAM antibody raised in Rabbit, conjugated with FITC. Designed to detect BCAM in Human for cardiovascular research.
Key elements and design rationale
- Immunogen: Recombinant Human Basal cell adhesion molecule protein (447-547AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- FITC: Direct fluorescence (Ex490/Em525); protect from light; use antifade.
Biological background
BCAM (also: Antigen identified by monoclonal F8 , AU, Auberger B antigen, B CAM cell surface glycoprotein, B cell adhesion molecule, B-CAM cell surface glycoprotein, Basal cell adhesion molecule (Lu and Au blood groups), Basal cell adhesion molecule (Lutheran blood group), Basal cell adhesion molecule, Basal cell adhesion molecule Lu and Au blood groups, Basal cell adhesion molecule Lutheran blood group, Bcam, BCAM_HUMAN, CD239, CD239 antigen, F8/G253 antigen, Glycoprotein 95kDa, LU, Lutheran, Lutheran antigen, Lutheran blood group (Auberger b antigen included), Lutheran blood group Auberger b antigen included , Lutheran blood group glycoprotein, MSK19) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current BCAM annotation relevant to cardiovascular.
Common research applications
- Refer to product documentation for confirmed application data.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
BCAM is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects BCAM in immunoassay applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Application data not specified. Consult datasheet or contact BioHippo support.
FITC (Ex490/Em525): direct fluorescence; protect from light; use antifade for sections.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing BCAM; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
