Bcl-2 Monoclonal Antibody(6B5)

SKU:BHA12104341
Overview
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BCL2-specific monoclonal antibody raised in Mouse. Validated for Immunofluorescence, Immunocytochemistry, Western Blot in Human, Mouse, Rat samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen Synthetic Peptide
Molecular Weight ~26.3 kDa
UniProt P10415
Gene ID Human: 596; Mouse: 12043; Rat: 24224
Applications 4 validated
Options selector
Catalog no. Size
BT-MCA0011-20UL 20 uL
BT-MCA0011-50UL 50 uL
BT-MCA0011-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-MCA0011
Alternative Names BCL2; Apoptosis regulator Bcl-2
Clonality
  • Monoclonal
Concentration 1 mg/ml
Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Gene ID Human: 596; Mouse: 12043; Rat: 24224
Host Mouse
Immunogen Synthetic Peptide of Bcl-2
Isotype
  • IgG
Molecular Weight ~26.3 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
  • Rat
  • Chicken
Storage -20°C for 1 year
Target BCL2

Product Overview

Primary Antibody

This antibody targets BCL2 and detects endogenous levels of BCL2 protein.

Validated Applications

Application Dilution Guidance
Immunofluorescence (IF) Refer to datasheet for recommended working dilution
Immunocytochemistry (ICC) Refer to datasheet for recommended working dilution
Western Blot (WB) Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC) Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target BCL2
Host Mouse
Clonality Monoclonal
Isotype IgG
Reactivity Human, Mouse, Rat, Chicken
Purification Affinity Purification
Molecular Weight ~26.3 kDa
Concentration 1 mg/ml
Immunogen Synthetic Peptide of Bcl-2
Formulation PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Storage -20°C for 1 year
Gene ID Human: 596; Mouse: 12043; Rat: 24224
UniProt P10415 (Human: P10415; Mouse: P10417; Rat: P49950)

Alternative Names

BCL2; Apoptosis regulator Bcl-2

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetBCL2
HostMouse
ClonalityMonoclonal
IsotypeIgG
ReactivityHuman, Mouse, Rat, Chicken
PurificationAffinity Purification
Molecular Weight~26.3 kDa
Concentration1 mg/ml
ImmunogenSynthetic Peptide of Bcl-2
FormulationPBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Storage-20°C for 1 year
Gene IDHuman: 596; Mouse: 12043; Rat: 24224
UniProtP10415 (Human: P10415; Mouse: P10417; Rat: P49950)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Immunofluorescence (IF)Refer to datasheet for recommended working dilution
Immunocytochemistry (ICC)Refer to datasheet for recommended working dilution
Western Blot (WB)Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC)Refer to datasheet for recommended working dilution

Alternative Names

BCL2; Apoptosis regulator Bcl-2

What species does this antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat, Chicken. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB), Immunohistochemistry (IHC). Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This monoclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of BCL2?
The predicted molecular weight of BCL2 is ~26.3 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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