| Field | Specification |
|---|---|
| Target | |
| Alternative names | Beclin-1|Coiled-coil myosin-like BCL2-interacting protein|Protein GT197|Beclin-1-C 35 kDa|Beclin-1-C 37 kDa|BECN1|GT197 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | beclin 1, autophagy related |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Beclin-1(BECN1), also known as ATG6 or VPS30, has a central role in autophagy, a process of programmed cell survival, which is increased during periods of cell stress and extinguished during the cell cycle. Beclin-1, may play a role in antiviral host defense. It protects against infection by a neurovirulent strain of Sindbis virus. Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. A progressively reduced Beclin-1 expression is reported to correlate with the primary tumor growth of squamous cell carcinoma and adenocarcinoma of the lung. Caspase-mediated cleavage of Beclin 1 promotes crosstalk between apoptosis and autophagy.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes BECN1. It is validated for ELISA, Immunohistochemistry, Western Blot, Immunofluorescence.
Validated Applications
| Applications | ELISA, Immunohistochemistry, Western Blot, Immunofluorescence |
|---|---|
| WB | 1:500-1:5000 |
| IHC | 1:20-1:200 |
| IF | 1:20-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
beclin 1, autophagy related
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | beclin 1, autophagy related |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 60 kDa |
| UniProt # | Q14457 |
| Gene ID | 8678 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Beclin-1|Coiled-coil myosin-like BCL2-interacting protein|Protein GT197|Beclin-1-C 35 kDa|Beclin-1-C 37 kDa|BECN1|GT197 |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for BECN1 research from Fine Test, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Metformin as a Modulator of Autophagy and Hypoxia Responses in the Enhancement of Wound Healing in Diabetic Rats. Inflammation (2024) [IF: 4.5] View Article
The histological investigation of the effects of electromagnetic radiation on rat ovaries. Journal of Molecular Histology (2024) [IF: 2.9] View Article
