| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized Powder |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min). |
| Source | Synthetic |
| Storage | |
| Target |
Product details
- Chemical name: 3,5-diamino-N-(N'-benzylcarbamimidoyl)-6-chloropyrazine-2-carboxamide.
- Also known as: Benzamil amiloride
- CAS number: 161804-20-2
- Molecular formula: C13H14ClN7O.
- Purity: >99%
- Concentration: 1-300 µM.
- Form: Lyophilized Powder
- Solubility: Up to 100 mM in DMSO. Centrifuge all products before use (10000 x g 5 min).
- Reconstitution: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Target: Na+/Ca2+ exchangers, ASIC/ENaC, TRPP3, TRPA1 channels
- Source: Synthetic
- Activity: Benzamil is a potent amiloride analogue, an inhibitor of the Na+/Ca2+ exchanger (IC50 = 0.1 µM)1, a blocker of Deg/ENaC channels2, of TRPP3 channels (IC50 = 1.1 µM)3 and of TRPA1 channels (IC50 = 55 µM)4.
- Alternative names: Benzamil amiloride
Scientific background
Benzamil is a potent amiloride analog that acts as an inhibitor of Na+/Ca2+ exchangers and a blocker of ASIC/Deg/ENaC, TRPP3, and TRPA1 channels displaying IC50 of 0.1, 1.1 and 55 µM respectively1-3.Benzamil is commercially available as an inhibitor for acid sensing ion channels (ASICs) belonging to the Deg/ENaC family. The compound is also well known as a diuretic compound that is used for the management of congestive heart disease or hypertension. Intrathecal administration of benzamil demonstrates antinociceptive effects in several pain models in animals and humans through the inhibition of acid sensing ion channels4.
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
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