| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
RARβ (retinoic acid receptor beta, RARB) is a ligand-activated nuclear receptor that mediates the effects of retinoic acid, a vitamin A derivative. RARβ heterodimerizes with the retinoid X receptor and binds retinoic acid response elements, typically arranged as DR5 repeats, to regulate target gene transcription in a ligand-dependent manner. The β-RARE element from the RARβ2 promoter is itself a classic retinoic-acid-inducible enhancer. RARβ is expressed in the retina and neuronal progenitors, where its activity, further modulated by the orphan receptor TLX, contributes to neuronal development, retinogenesis, and neurogenesis. Reporter systems based on β-RARE elements provide a quantitative readout of retinoic acid signaling through RARβ.
Product Description & Applications
The β-RARE Reporter Lentivirus is a transcription-factor reporter system for detecting RARβ-mediated transcriptional activity in mammalian cells. The construct uses tandem repeats of RARβ DNA-binding elements in the DR5 β-RARE configuration from the RARβ2 promoter, coupled to a minimal promoter that drives a fluorescent or luminescent reporter, to preferentially support activation by RARβ. A constitutively expressed selection marker and optional secondary reporter allow generation of stable polyclonal reporter cell lines.
Stable lentiviral integration provides consistent reporter expression in dividing and post-mitotic cells, including primary and cryopreserved cultures, avoiding transient-transfection variability. Particles are purified by PEG precipitation and sucrose gradient centrifugation and transduce difficult-to-transfect cells, supporting research on retinoic acid signaling in neuronal development and retinogenesis, with readout by microscopy, flow cytometry, or luminometry.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the RAR-b Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
