Beta-TC-6 cell

SKU:BHC11101154
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Overview
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Beta-TC-6 cell is a cell line. It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Epithelial. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Mouse
Disease model Mouse insulinoma
Morphology Epithelial
Growth Properties Adherent
Tissue Pancreas
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Catalog no. Size
305181 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305181
Species Mouse
Beta-TC-6 cells is a cell line derived from insulinoma tissue in mice. These cells are crucial in scientific studies focused on diabetes and insulin signalling. Originating from a transgenic mouse, Beta-TC-6 cells carry a pseudogene construct comprising the SV40 early region, which the rat insulin gene promoter regulates. This genetic composition leads to insulin secretion in response to glucose levels. These cells exhibit epithelial morphology and primarily reside in the pancreas tissue. In addition to insulin production, these cells possess small amounts of glucagon and somatostatin. The adherence of Beta-TC-6 cells allows for convenient cultivation and manipulation during experiments and assays. Beta-TC-6 cells provide a valuable tool for scientific investigations in diabetes and insulin signalling. Their unique genetic composition, insulin-secreting capabilities, and adherence properties make them ideal for studying the intricate processes involved in glucose regulation and pancreatic function.

SKU:BHC11101154

  • cultureMedium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
  • supplements: Supplement the medium with 15% heat-inactivated FBS
  • dissociationReagent: Accutase
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • fluidRenewal: 2 to 3 times per week
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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