| Field | Specification |
|---|---|
| Target | |
| Alternative names | Tubulin beta-3 chain|Tubulin beta-4 chain|Tubulin beta-III|TUBB3|TUBB4 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | tubulin, beta 3 |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha and beta tubulins have been widely used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
Product Overview
This monoclonal antibody (clone 8A10), raised in Mouse, recognizes Beta Tubulin. It is validated for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
|---|---|
| WB | 1:10000-1:50000 |
| IF | 1:20-1:200 |
| IP | 1:500-1:2000 |
| IHC | 1:50-1:500 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
tubulin, beta 3
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | 8A10 |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | tubulin, beta 3 |
| Purification | Protein A+G Purification |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 50 kDa |
| UniProt # | Q13509 |
| Gene ID | 10381 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Tubulin beta-3 chain|Tubulin beta-4 chain|Tubulin beta-III|TUBB3|TUBB4 |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for Beta Tubulin research from Fine Test, or contact us for alternative formats.
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