| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C21H19ClN4O |
| SMILES | |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
BF844 mitigates hearing loss associated with the USH3 (Usher syndrome type III) CLRN1 (clarin-1) N48K mutation by inducing CLRN1 N48K to transport to the plasma membrane, and it significantly preserves hearing in vivo[1]. It has a molecular formula of C21H19ClN4O and a molecular weight of 378.85 g/mol.
Physical & Chemical Properties
| CAS Number | 1404506-35-9 |
|---|---|
| Molecular Formula | C21H19ClN4O |
| Molecular Weight | 378.85 g/mol |
| SMILES | CC(O)(C)CN1N=C(C2=CC=CC=C2)C3=C(Cl)C(C4=CC=CC=C4)=NN=C31 |
| Signaling Pathway | Cell Cycle/DNA Damage; Metabolic Enzyme/Protease |
| Storage | Please store the product under the recommended conditions in the Certificate of Analysis. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with your institution's chemical hygiene plan.
In Vitro
BF844 at 0.846 μM effectively inhibits HSP60 activity (87.07±27.70% inhibition) and moderately inhibits HSP90 (40.06±19.10% inhibition)[1]. In C1, D1, D6 cells, BF844 (2.90 μM; 24 h) causes about 6% of total CLRN1N48K to be transported to the plasma membrane[1]. In C1 and D1 cells, BF844 (2.90 μM; 24 h) effectively increases the amount of non-glycosylated CLRN1, and this non-glycosylated CLRN1 is effectively transported to the plasma membrane[1].
In Vivo
In vivo, BF844 penetrates the retina and cochlea well[1]. In Tg;KI/KI mice, BF844 (10 mg/kg; i.p.) significantly preserves hearing[1].
| Animal Model | Juvenile mice[1] |
|---|---|
| Dosage | 3, 10 mg/kg (3 mg/kg for P6 (post-natal day 6) mice, 10 mg/kg for P20 (post-natal day 20) mice) |
| Administration | I.p. |
| Result | Showed AUC values were determined to be 1.76 μM.h and 1.98 μM.h. |
| Animal Model | P30 Tg;KI/KI C57BL/6J mice[1] |
|---|---|
| Dosage | 30 mg/kg |
| Administration | I.p.; daily from P30 to P45 |
| Result | Showed significantly preserves hearing with the median threshold of sound intensity in log scale was 57.5–67.5 dB SPL and about 1,000 times more sensitive hearing compared to untreated controls at P55. |
| Animal Model | P10 Tg;KI/KI C57BL/6J mice[1] |
|---|---|
| Dosage | 10 mg/kg |
| Administration | I.p.; 10 mg/kg every other day and gradually escalated the dose up to 20 mg/kg at P28. From P30 to P45, mice received 30 mg/kg daily |
| Result | Showd the median threshold of sound intensity in log scale was 55, 42.5 and 37.5 dB SPL lower at 8, 16 and 32 kHz at P55, respectively. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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