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The BHT101 cell line is derived from the lymph node metastasis of a 63-year-old woman diagnosed with anaplastic papillary thyroid carcinoma. This cell line is established from a highly aggressive and lethal form of thyroid cancer, known for its rapid progression and poor prognosis. BHT101 cells are notable for their lack of hormone production, which is typical for cells originating from anaplastic thyroid carcinoma, as these cells often lose the ability to synthesize thyroid hormones that are characteristic of more differentiated thyroid tissues.
In terms of biomarker expression, BHT101 cells are partially positive for thyroglobulin and thyroxine (T4). Thyroglobulin is a precursor glycoprotein critical for the production of the thyroid hormones T3 and T4 and is commonly used as a tumor marker in differentiating thyroid cancer types. The presence of thyroglobulin in BHT101 cells, even if only partial, is significant for research focused on thyroid cancer pathology and the molecular mechanisms underlying dedifferentiation in thyroid carcinomas. This cell line's unique profile makes it a valuable model for studying the progression and metastatic behavior of anaplastic thyroid carcinoma, providing insights into the molecular alterations that drive these processes.
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- cultureMedium: MEM (We do not supply this product; please consider other suppliers. Please let us know if you need further assistance)
- supplements: Supplement the medium with 20% heat-inactivated FBS, 5 microgram/mL human insulin, 0.005 IU/mL TSH (from Scrippslabs) - Add the required TSH just before use and sterile filter into the medium
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- fluidRenewal: 2 to 3 times per week
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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