| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C23H25N3O3 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
BI 99179, a chemical probe, is a potent, selective inhibitor of type I fatty acid synthase (FAS), with an IC50 of 79 nM. As a tool compound, it is suited to in vivo validation of FAS as a target in lipid-metabolism-related disease, and it shows substantial peripheral and central exposure after oral dosing in rats[1][2]. It is supplied as a white to off-white solid (C23H25N3O3, MW 391.46) at 99.38% purity.
Physical & Chemical Properties
| CAS Number | 1291779-76-4 |
|---|---|
| Molecular Formula | C23H25N3O3 |
| Molecular Weight | 391.46 g/mol |
| Purity | 99.38% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | O=C([C@H]1C[C@@H](NC(CC)=O)CC1)N(C2=CC=C(C3=NC4=CC=CC=C4O3)C=C2)C |
| Signaling Pathway | Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: 33.33 mg/mL (85.14 mM; Requires sonication and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
IC50: 79 nM (FASN)[1]
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 33.33 mg/mL (85.14 mM) | requires sonication and warming and heat to 60°C; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | ≥ 2.08 mg/mL (5.31 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.08 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (20.8 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | ≥ 2.08 mg/mL (5.31 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.08 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (20.8 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Protocol 3
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 2.08 mg/mL (5.31 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.08 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (20.8 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
In mouse hypothalamic N-42 cells, BI 99179 is potent, with an IC50 of 0.6 μM. In the cytotoxicity assay, BI 99179 produces no significant LDH release at concentrations up to 30 μM[1]. In human glioma GAMG cells, BI 99179 (BI-99179; 1, 2, and 4 μM) displays antiproliferative efficacy[2].
Cell Proliferation Assay[2]
| Cell Line | Human glioma GAMG cells |
|---|---|
| Concentration | 1, 2, 4 μM |
| Incubation Time | 96 to 120 hours |
| Result | The optimal palmitate concentration for GAMG cell line was 4 μM. |
In Vivo
In male Han/Wistar rats (oral application of 4 mg/kg), BI 99179 has a super pharmacokinetic profile, with a half life (t1/2) of 3.0 h[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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