| Field | Specification |
|---|---|
| Target | |
| Alternative names | Apoptic death agonist; Apoptotic death agonist BID; BH3 interacting domain death agonist; BH3 interacting domain death agonist p11; BH3 interacting domain death agonist p13; BH3 interacting domain death agonist p15; BH3-interacting domain death agonist p11; BID; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID_HUMAN; Desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p11 BID; p13 BID; p15 BID; p22 BID |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human BH3-interacting domain death agonist protein (1-195AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This is a polyclonal anti-BID antibody raised in Rabbit, conjugated with HRP, with confirmed utility in ELISA. Designed to detect BID in Human, supporting researchers in cell biology contexts.
Key elements and design rationale
- Immunogen: Recombinant Human BH3-interacting domain death agonist protein (1-195AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.
- Host (Rabbit): Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.
- Polyclonal: Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.
- Isotype (IgG): Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A/G compatibility.
- Purification (Protein G purification): Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.
- HRP conjugate: Direct chromogenic (TMB/OPD) or chemiluminescent (ECL) detection. Quench endogenous peroxidase in tissue applications. No secondary required.
Biological background
BID (also known as Apoptic death agonist, Apoptotic death agonist BID, BH3 interacting domain death agonist, BH3 interacting domain death agonist p11, BH3 interacting domain death agonist p13, BH3 interacting domain death agonist p15, BH3-interacting domain death agonist p11, BID, BID isoform ES(1b), BID isoform L(2), BID isoform Si6, BID_HUMAN, Desmocollin type 4, FP497, Human BID coding sequence, MGC15319, MGC42355, p11 BID, p13 BID, p15 BID, p22 BID) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of BID biology relevant to cell biology.
Common research applications
- ELISA: Sandwich or indirect ELISA quantifies soluble/secreted target in biological fluids or cell culture supernatants.
Notes for experimental interpretation
- Isotype controls: Include IgG from Rabbit at equivalent concentration to assess non-specific background.
- Cross-reactivity/isoforms: Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.
- Matrix effects: Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.
- Species reactivity: Confirmed for Human; extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
BID is a protein target in Human biology. This polyclonal antibody raised in Rabbit detects BID and is validated for ELISA applications.
This antibody is reported reactive against Human. Immunogen derived from Human. Cross-reactivity with unlisted species should not be assumed — contact us or consult the datasheet for homology data.
Supplier reports performance in ELISA. Dilution optimization, blocking, and appropriate controls are required per format. Contact BioHippo for recommended working concentrations.
HRP enables direct chromogenic (TMB/OPD) or chemiluminescent (ECL) detection — no secondary needed. Block endogenous peroxidase in tissue applications before applying this antibody.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — lysate/cell line expressing BID; (3) Negative control — siRNA KD or KO sample to confirm specificity; (4) Dilution linearity — verify proportional signal decrease for quantitative applications.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
