| Field | Specification |
|---|---|
| Mfr No | |
| Form | 2X Mastermix |
| Product Type | |
| Storage |
Blast through a routine 2 hour PCR run in only 20 minutes!
BlasTaq™ 2X PCR MasterMix is a ready-to-use MasterMix containing abm’s BlasTaq™ DNA Polymerase in a uniquely-formulated buffer with gel loading dye. BlasTaq™ DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates and robust performance. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors.
Product Features:
- 6 kb/min extension speed
- 40-70% less time needed than reguar Taq
- Superior senstivitiy
- Available as a standalone enzyme (Cat. No. G894)
- Included in abm's PCR Buffet Program
| Product Component | Quantity |
|---|---|
| BlasTaq™ 2X PCR MasterMix1 | 800 rxn (10.0 ml) |
1 MasterMix contains 1.5 mM Mg2+
 |
ISO 13485:2016 MDSAP Certified |
| Component | Quantity |
|---|---|
| BlasTaq™ 2X PCR MasterMix | 800 rxn (10.0 ml) |
| Specification | Value |
|---|---|
| Enzyme Type | PCR Mastermix |
| Format | 2X Mastermix |
| Hot Start | No |
| Fidelity | Standard |
| Extension Speed | 6 kb/min |
| 3'→5' Proofreading | No |
| Mg²⁺ Concentration | 1.5 mM |
| Storage Conditions | Store at -20°C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
The loading dye does not interfere with downstream cloning experiments. However, PCR clean-up is still advised prior to cloning due to the presence of excess primers, salts, and other buffer components that could potentially affect cloning efficiency.
BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
Yes, BlasTaq™ DNA Polymerase produces 3’-dA-tailed amplicons, which makes it compatible with TA cloning vectors.
Yes, BlasTaq™ is designed to handle a variety of templates, including those with high GC content. For high-GC templates, it is recommended to increase the initial denaturation step to 5 minutes to ensure complete denaturation of the template.
BlasTaq™'s specialized buffer is optimized for most PCR applications, including primer annealing at 60°C. However, if your primers do not efficiently anneal or if you are working with particularly challenging templates, you may need to optimize the annealing temperature or adjust cycling conditions based on your results.
It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
The shelf life of BlasTaq™ is typically indicated on the product label or datasheet, but as a general rule, it is stable for up to 1-2 years when stored properly at -20°C. Be sure to check the expiration date and make sure the enzyme remains frozen when not in use.
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