| Field | Specification |
|---|---|
| Detection Method | |
| Form | 2X Mastermix |
| Product Type | |
| Storage |
BlasTaq™ 2X qPCR MasterMix provides a convenient, reliable, and robust solution for performing quantitative real-time PCR (qPCR) analysis of DNA samples. This ready-to-use MasterMix contains BlasTaq™ DNA Polymerase, a next-generation Taq polymerase strategically engineered by abm to deliver faster extension rates and stronger overall performance.
With optimized reaction conditions, BlasTaq™ DNA Polymerase offers significantly improved processivity, higher yields, and enhanced sensitivity. These improvements allow for faster reaction times, shortening qPCR runs by up to 70% compared to reactions using wild-type Taq polymerase. BlasTaq™ possesses 5’-3’ polymerase activity and 5’-3’ exonuclease activity, but lacks 3’-5’ exonuclease activity. It also produces 3’-dA-tailed amplicons, making qPCR products generated with BlasTaq™ compatible with TA cloning systems.
The MasterMix contains a fluorescent dye compatible with commonly used detection systems, such as SYBR Green™ and EvaGreen™. Additionally, ROX Reference Dye is supplied separately, ensuring compatibility with most qPCR instruments. For specific instrument requirements, please refer to the ROX Machine Compatibility Chart.
Product Features:
- Enable streamlined protocol in a simple reaction set-up
- Allow accurate quantification of a variety of gene targets
- Reduce pipetting steps to minimize the risk of contamination
- Compatible with most real-time PCR instruments
- Contains dye comparable to SYBR Green™ and EvaGreen™
(Probe-based qPCR Master Mix also available) - Included in abm's PCR Buffet Program
For a larger size, see Cat. No. G892 (2,500 rxn, 25.0 ml)
| Product Component | Quantity |
|---|---|
| BlasTaq™ 2X qPCR MasterMix | 500 rxn (4 x 1.25 ml) |
| ROX Reference Dye | 50 µl |
|
Customer Testimonial "Very nice product. I always get consistent results. Even low expression genes can be quantified easily. Compatible with both standard and fast reaction protocols." - Dr. Vivek Vaish, Pune University |
 |
ISO 13485:2016 MDSAP Certified |
||||||||
| Concentration | 2X | ||||||||
| Storage Condition |
Store at -20 °C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
||||||||
| Note |
BlasTaq™ 2X qPCR MasterMix includes a separate vial of ROX Reference Dye. You can add this dye if your specific qPCR machine requires ROX, as indicated in the table below:
|
| Component | Quantity |
|---|---|
| BlasTaq™ 2X qPCR MasterMix | 500 rxn (4 x 1.25 ml) |
| ROX Reference Dye | 50 µl |
| Specification | Value |
|---|---|
| Enzyme Type | qPCR Mastermix |
| Format | 2X Mastermix |
| 3'→5' Proofreading | No |
| Storage Conditions | Store at -20 °C. This product is stable for 2 years from the date of shipping if stored and handled properly. |
For further details, please email [contact ABM technical support] or 1-866-757-2414.
Yes, it is comparable.
It is a ready-to-use qPCR MasterMix in which only template and primers need to be added by the end user. BlasTaq™ has super speed performance to achieve results less than a hour. ROX Reference Dye is offered on the side to give more flexibility to the user for your qPCR set up.
BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
abm's qPCR MasterMixes don't contain ROX dye, but it is provided in a separate tube when you purchase any abm qPCR MasterMix. Some qPCR machines may require ROX, so please consult our machine compatibility list on the product page to see if your instrument needs it.
The recommended volume of ROX reference dye varies based on the qPCR instrument: No ROX equipment: No dye needed. Low ROX equipment: 1 μl/1.25 ml or 22.5 μl/25 ml. High ROX equipment: 11.5 μl/1.25 ml or 225 μl/25 ml.
Yes, this MasterMix is universally compatible with most qPCR instruments, as the ROX dye is provided separately for flexibility.
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