| Field | Specification |
|---|---|
| Mfr No | |
| Form | 2X Mastermix |
| Product Type | |
| Storage |
BlasTaq™ HotStart 2X PCR MasterMix is a convenient, ready-to-use solution containing abm’s BlasTaq™ HotStart DNA Polymerase in a specially formulated buffer with gel loading dye. This advanced Taq polymerase is engineered for rapid extension rates, robust performance, and includes a proprietary antibody that prevents the polymerase from working at low temperatures. The HotStart feature allows for easy reaction set-up at room temperature, preventing non-specific amplification and primer dimer formation.
BlasTaq™ HotStart offers enhanced processivity, higher yields, increased sensitivity, and significantly reduced reaction times—up to 70% faster than traditional wild-type Taq polymerase. During the initial denaturation, the antibody releases from the polymerase, restoring enzyme activity and reducing non-specific amplification, which ensures higher specificity and improved PCR product yields. BlasTaq™ also features 5’-3’ polymerase and exonuclease activities, but lacks 3’-5’ exonuclease activity, producing 3’-dA-tailed amplicons that are compatible with TA cloning vectors.
Product Features:
- No non-specific amplification or primer-dimers
- Improves yield of desired product
- Simplifies reaction setup
- Included in abm's PCR Buffet Program
 |
ISO 13485:2016 MDSAP Certified |
Please refer to the product page for component information.
| Specification | Value |
|---|---|
| Enzyme Type | PCR Mastermix |
| Format | 2X Mastermix |
| Hot Start | Yes |
| Fidelity | Standard |
| 3'→5' Proofreading | No |
| Storage Conditions | Store at -20°C. |
Each lot is functionally tested to verify activity prior to release. A Certificate of Analysis (CoA) documenting lot-specific quality control data is available on request.
Store at −20°C in a non-frost-free freezer. Avoid repeated freeze-thaw cycles; aliquoting for long-term storage is recommended. This product is stable for ≥2 years from the date of shipment under proper storage conditions.
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