| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Storage |
Eliminate non-specific amplification and primer-dimer formation!
BlasTaq™ HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase.
During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors.
Product Features:
- No non-specific amplification or primer-dimers
- Improves yield of desired product
- Simplifies reaction setup
- Available as a MasterMix (Cat. No. G598)
- Included in abm's PCR Buffet Program
| Product Component | Quantity |
|---|---|
| BlasTaq™ HotStart DNA Polymerase | 400 rxn (200 µl) |
| 5X BlasTaq™ Buffer 1 | 2 x 1.0 ml |
1 Buffer contains 1.5 mM Mg2+.
 |
ISO 13485:2016 MDSAP Certified |
| Specification | Value |
|---|---|
| Enzyme Type | DNA Polymerase |
| Format | Enzyme Only |
| Hot Start | Yes |
| Fidelity | Standard |
| 3'→5' Proofreading | No |
| Mg²⁺ Concentration | 1.5 mM |
| Storage Conditions | Store at -20°C. |
This product is abm’s next generation of PCR enzymes and is functionally equivalent to Cat. No. G011 and G039 (HotStart DNA Polymerase) as well as Cat. No. G937 (SensTaq HotStart DNA Polymerase), with improved performance. Contact our customer service team [contact ABM technical support] for more information.
BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
Yes, BlasTaq™ DNA Polymerase produces 3’-dA-tailed amplicons, which makes it compatible with TA cloning vectors.
If you’re working with difficult targets or crude samples, it may be necessary to increase the amount of enzyme to 1 μL for better performance. Additionally, for templates with high GC content, you can increase the initial denaturation step to 5 minutes.
Yes, BlasTaq™ is designed to handle a variety of templates, including those with high GC content. For high-GC templates, it is recommended to increase the initial denaturation step to 5 minutes to ensure complete denaturation of the template.
BlasTaq™'s specialized buffer is optimized for most PCR applications, including primer annealing at 60°C. However, if your primers do not efficiently anneal or if you are working with particularly challenging templates, you may need to optimize the annealing temperature or adjust cycling conditions based on your results.
It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
References
- Feride Ş; Rugıyya S; Hüseyin K et al.. Investigation of Blastocystis spp. in patients with inflammatory bowel disease by direct microscopy and molecular methods. African health sciences. 2025;25:74-83. DOI
- Altay K; Erol U; Sahin OF et al.. Application of LAMP and TaqMan qPCR for the rapid diagnosis of Anaplasma Capra (an emerging tick-borne zoonotic pathogen) and comparison with Nested-PCR. Veterinary research communications. 2026;50:153. DOI
- Batur LK; Yavas C; Polat Y. Investigating the potential selective effects of a Kiperin multi-component magnesium supplement on colon cancer and normal cells. Scientific reports. 2025;16:2988. DOI
