| Field | Specification |
|---|---|
| Alternative Names | BUN, Blood urea nitrogen |
| Assay Type | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, Plasma, Urine, Saliva, Tissue Culture Media |
| Sensitivity | |
| Shipping | |
| Species | |
| Storage | |
| Target |
Scientific Background
Urea is the primary nitrogen-containing excretory metabolite of protein catabolism, formed in the liver via the ornithine cycle (urea cycle) from ammonium ions generated by amino acid deamination and from arginine metabolism. Blood urea nitrogen (BUN) quantifies the urea fraction of total blood nitrogen; normal adult reference values are 7–21 mg/dL, with BUN:creatinine ratios further distinguishing pre-renal from intrinsic renal causes of azotemia.
In neuroscience and nephrology research, BUN elevation reflects impaired glomerular filtration or augmented protein catabolism associated with systemic stress, sepsis, or corticosteroid administration. Uremic retention solutes—including urea itself—have been mechanistically linked to neuroinflammation, disruption of the blood–brain barrier, and cognitive dysfunction in chronic kidney disease (CKD) patients, a syndrome now termed CKD-associated neurological complications.
Non-invasive BUN measurement in urine or saliva, combined with creatinine normalization, provides a practical tool for monitoring renal metabolic clearance in longitudinal neurological studies without venipuncture.
Product Description
StressXpress®
The Blood Urea Nitrogen (BUN) Detection Kit (Cat. # SKT-213) from StressMarq Biosciences is a research-grade kit for quantitative Urea Nitrogen measurement in Serum, Plasma, Urine, Saliva, Tissue Culture Media. This kit employs a direct colorimetric detection principle with colorimetric TMB/spectrophotometric readout on a standard 96-well microplate reader.
Product Specifications
| Analyte / Target | Urea Nitrogen |
|---|---|
| Assay Type | Direct Quantitative Assay |
| Detection Method | Colorimetric (microplate spectrophotometry) |
| Sensitivity | 0.042 mg/dl |
| Detection Range | 0.156–10 mg/dl |
| Compatible Matrices | Serum, Plasma, Urine, Saliva, Tissue Culture Media |
| Species Reactivity | Species Independent |
| Throughput | 88 samples in duplicate |
| Incubation Time | 30 Minutes |
| Platform | Microplate (96-well) |
| Catalog Number | SKT-213 |
| Storage | 4ºC and -20ºC |
| Trademark | StressXpress® |
Assay Overview
This kit uses a direct colorimetric enzymatic reaction for quantitative Urea Nitrogen measurement without the need for sample pre-extraction. A calibrated standard (traceable to NIST reference material where applicable) is provided to construct a standard curve for interpolation. Results are read on a standard microplate spectrophotometer.
Compatible sample matrices include: Serum, Plasma, Urine, Saliva, Tissue Culture Media.
Kit Components
| Component # | Description | Quantity / Volume |
|---|---|---|
| SKC-213A | Clear 96-Well Plates | 2 Plates |
| SKC-213B | Urea Nitrogen Standard | 250 µl |
| SKC-213C | Color Reagent A | 15 ml |
| SKC-213D | Color Reagent B | 15 ml |
| SKC-213E | Urease Enzyme | 2 vials |
Quality Control & Validation
✓ Peer-Reviewed & Research Validated
Each lot is validated for sensitivity, intra-assay precision (CV <10%), and linearity of recovery. Products from the StressXpress® range cite published peer-reviewed literature for independent validation.
Safety & Regulatory
ADR Classification: Non-hazardous. Shipping: cold-chain (blue ice). Harmonized Tariff Code: 3822.19.0030 (UNSPSC: 12352203). Country of Origin: Canada.
This colorimetric assay is validated for use with: Serum, Plasma, Urine, Saliva, Tissue Culture Media. Samples should be diluted with the supplied Assay Diluent or deionized water as specified in the protocol. Lipemic, highly pigmented, or protein-rich samples may require additional dilution to minimize matrix interference.
The kit has a sensitivity of 0.042 mg/dl and a quantification range of 0.156–10 mg/dl. This range is appropriate for typical physiological concentrations. Samples outside the range should be diluted (upper) or concentrated using ultrafiltration (lower).
Total assay time including incubation is approximately 30 Minutes. Refer to the Kit Booklet for the detailed protocol and timing requirements.
The kit contains: Clear 96-Well Plates, Urea Nitrogen Standard, Color Reagent A, Color Reagent B, Urease Enzyme. All reagents are pre-formulated and lot-matched. A calibrated creatinine or nitrogen standard is included for standard curve generation.
A standard 96-well microplate spectrophotometer capable of reading absorbance at the target wavelength (typically 490–550 nm for colorimetric reactions; see Kit Booklet for exact wavelength) is required. The assay is not compatible with a fluorescence reader.
Urine and tissue culture supernatant samples should be diluted with deionized water or assay diluent as specified in the protocol (typically 1:20 for urine). Serum/plasma samples may be used undiluted or at minimal dilution. No extraction step is required. Sample creatinine values are reported in mg/dL; normalize to urine volume or creatinine concentration as appropriate for your experimental design.
This product may be available in custom quantities, alternative formulations, or with additional quality certifications to meet specific experimental requirements. Contact our scientific team through the BioHippo product page to discuss custom sizing, bulk ordering, and specialized packaging options. Special pricing may be available for volume orders and institutional customers.
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