| Field | Specification |
|---|---|
| Applications | |
| Molecular weight | |
| Molecular formula | C21H16N2O6 |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
BMP agonist 1 is a small-molecule agonist of bone morphogenic protein (BMP). BMP-induced C2C12 cell differentiation is highly dependent on active BMP signaling; BMP agonist 1 inhibits GSK3β, increases β-catenin signaling, and synergistically regulates Id2 and Id3 expression, and it is used in research on diseases and defects of the skeleton[1]. It is supplied as an orange to red solid (C21H16N2O6, MW 392.36).
Physical & Chemical Properties
| Molecular Formula | C21H16N2O6 |
|---|---|
| Molecular Weight | 392.36 g/mol |
| Appearance | Solid |
| Color | Orange to red |
| SMILES | OC1=CC2=C(C=C1)NC3=C2C(C(N4)=O)=C(C5=CC(OC)=C(C(OC)=C53)OC)C4=O |
| Signaling Pathway | TGF-beta/Smad; Stem Cell/Wnt; PI3K/Akt/mTOR |
| Solubility | In Vitro: DMSO: 50 mg/mL (127.43 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 50 mg/mL (127.43 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
BMP agonist 1 (0.01-10 μM, 24 h) lowers the proliferation of C2C12 cells[1]. In C2C12 cells, BMP agonist 1 (0.5-1 μM, 2 h) inhibits GSK3β, increases β-catenin signaling, and acts synergistically to regulate Id2 and Id3 expression[1]. BMP agonist 1 (0.01-10 μM) potently promotes osteogenic differentiation (Alp activity) driven by the canonical BMP potentiator Chromenone 1 in C2C12 cells when used together with Chromenone 1 (0.5−1 μM)[1].
Cell Proliferation Assay[1]
| Cell Line | C2C12 cells |
|---|---|
| Concentration | 0.01-10 μM |
| Incubation Time | 24, 72 h |
| Result | Differentiaed in C2C12 osteoblast at the low-dose BMP-4 (7.5 ng/mL). Reduced the cell numbers and stopped the proliferation at higher concentrations. Highly induced Id1 and Id3, elevated Runx2 levels. |
Cell Differentiation Assay[1]
- Cell Line: C2C12 cells
- Concentration: 0.01-10 μM (in combination with (0.05, 0.2, 0.5, or 1 μM Chromenone 1)
- Incubation Time:
- Result: Highly overadditive effects and increased potency with Chromenone 1. Reached the highest synergy scores (i.e., delta scores) at a concentration of 0.3 μM with 0.5−1 μM Chromenone 1.
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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