| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C19H26ClN3O4 |
| Purity | |
| SMILES | |
| Form | Solid-Liquid Mixture |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
BN82002 hydrochloride is a potent, selective, irreversible inhibitor of the CDC25 phosphatase family. It inhibits CDC25A, CDC25B2, CDC25B3, CDC25C CDC25A, and 25C-cat, with IC50 values of 2.4 μM, 3.9 μM, 6.3 μM, 5.4 μM and 4.6 μM, respectively, and shows roughly 20-fold greater selectivity over CD45 tyrosine phosphatase[1]. It is supplied as a brown to orange solid-liquid mixture (C19H26ClN3O4, MW 395.88) at 99.36% purity.
Physical & Chemical Properties
| CAS Number | 1049740-43-3 |
|---|---|
| Molecular Formula | C19H26ClN3O4 |
| Molecular Weight | 395.88 g/mol |
| Purity | 99.36% |
| Appearance | Solid-Liquid Mixture |
| Color | Brown to orange |
| SMILES | O=[N+]([O-])C1=CC=C(CCN(CC2=CC(N(C)C)=CC(OC)=C2O)C)C=C1.Cl |
| Signaling Pathway | Metabolic Enzyme/Protease |
| Solubility | In Vitro: DMSO: 220 mg/mL (555.72 mM; Requires sonication; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | 4°C, sealed storage, away from moisture and light. In solvent: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
IC50 & Target
IC50: 2.4 μM (CDC25A), 3.9 μM (CDC25B2), 6.3 μM (CDC25B3), 5.4 μM (CDC25C), 4.6 μM (CDC25C-cat)[1].
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 220 mg/mL (555.72 mM) | requires sonication; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); sealed storage, away from moisture and light; avoid repeated freeze-thaw cycles.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | ≥ 5.5 mg/mL (13.89 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 5.5 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (55.0 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | ≥ 5.5 mg/mL (13.89 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 5.5 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (55.0 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Protocol 3
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | 5.5 mg/mL (13.89 mM); suspension; requires sonication |
| How to prepare | Gives a suspension at 5.5 mg/mL. The suspension is suitable for oral and intraperitoneal dosing. For 1 mL of working solution: add 100 μL DMSO stock (55.0 mg/mL) to 900 μL corn oil. |
Data provided by the manufacturer.
In Vitro
Cell proliferation is evaluated in vitro on several human tumor cell lines to determine the effect of BN82002, with Menadione, which has been reported to inhibit cell proliferation, as a control. All examined cell lines are sensitive to both BN82002 and Menadione, in a concentration-dependent manner within the low micromolar range. MIA PaCa-2 pancreatic cancer cells are the most sensitive (IC50 of 7.2 μM), and HT-29 colon cancer cells are the least sensitive (IC50 of 32.6 μM). This range of activity closely resembles that of menadione (5-15 μM). It is also shown that BN82002 at 50 μM fully inhibits cell proliferation while the cell cycle distribution is only modestly affected, showing a slight decrease in S phase together with a rise in the number of cells with both a G1 and a G2 DNA content, which suggests that BN82002-treated cells are arrested at various stages of the cell cycle[1].
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
Need this compound in a format that drops straight into your assay? We can tailor formulation, chemistry, and documentation so your results stay consistent across runs and re-orders.
- Format options: solid or pre-dissolved solution (choose solvent), target concentration, aliquots, light/moisture-protected packaging
- Chemistry options: free base/acid vs salt forms, hydrate/solvate preference, stereoisomer control (single enantiomer or racemate), close analogs
- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
- QC & documentation: standard COA or enhanced analytical pack (HPLC/LC-MS/NMR), chiral purity, residual solvents, water content (KF), method-specific specs
- Scale & continuity: mg to gram scale, bulk pricing, lot reservation, repeat-order continuity
To quote quickly, tell us: compound name + CAS/structure (SMILES or mol file), intended assay context, solvent preference, salt/stereochemistry requirements, purity/QC level, and the amount (mg–g).
Can’t find the compound you’re looking for?
Send the CAS or structure and your specs. We can help source it, suggest close equivalents, or discuss custom synthesis with the right QC documentation (RUO).
Subunit P60 of phosphatidylinositol 3-kinase promotes cell proliferation or apoptosis depending on its phosphorylation status. PLoS Genet 2021 Apr 26;17(4):e1009514. PMID: 33901186
