| Field | Specification |
|---|---|
| Alternative Names | 14-3-3; 1C5; HS1; 14-3-3 protein T-cell|14-3-3 protein tau|14-3-3 protein theta|OTTHUMP00000165887|protein tau|tyrosine 3/tryptophan 5 -monooxygenase activation protein; theta polypeptide |
| Product Type | |
| Sensitivity | |
| UniProt # |
Product Overview
Sandwich ELISA KitYWHAq product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the dise…
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate YWHAQ in samples. An antibody specific for YWHAQ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYWHAQ present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YWHAQ is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avid…
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Method | Sandwich ELISA |
| Detection Range | 0.156-10 ng/mL |
Stability & Storage
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Bio…
Safety & Handling
Related Products
Browse our complete selection of ELISA kits, antibodies, and assay reagents across species at BioHippo.
This is a sandwich ELISA kit. It employs a capture antibody pre-coated on the microplate surface and a biotin-conjugated detection antibody. After incubation with streptavidin-HRP and TMB substrate, a colorimetric signal proportional to analyte concentration is generated and measured at 450 nm.
The linear detection range of this kit is 0.156-10 ng/mL. Samples exceeding the upper limit must be diluted prior to testing. Samples below the lower limit may require concentration steps. Always verify that interpolated values fall within the linear portion of the standard curve.
This kit is validated for use with serum and plasma samples. Cell culture supernatants and tissue homogenates may also be tested after appropriate preparation. Avoid grossly hemolyzed, lipemic, or bacterially contaminated samples, as these may produce inaccurate results.
A microplate reader capable of measuring absorbance at 450 nm (with a 540–570 nm reference wavelength) is required. A plate washer is recommended for consistent washing steps. Calibrated micropipettes, incubator (37°C), and standard laboratory consumables are also needed.
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O… Prepare working reagents fresh for each run. Do not freeze kit components unless specifically directed in the insert.
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