| Field | Specification |
|---|---|
| Target | |
| Alternative names | LPP2; PAP-2c; PAP2-g; lipid phosphate phosphohydrolase 2|phosphatidic acid phosphohydrolase type 2c|type-2 phosphatidic acid phosphatase-gamma |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Lipid phosphate phosphohydrolase 2 is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is similar to phosphatidic acid phosphatase type 2A (PPAP2A) and type 2B (PPAP2B). All three proteins contain 6 transmembrane regions, and a consensus N-glycosylation site. Phosphatidic Acid Phosphatase Type 2C has been shown to possess membrane associated PAP activity. Three alternatively spliced transcript variants encoding distinct isoforms have been reported.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate PPAP2C in samples. An antibody specific for PPAP2C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPAP2C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPAP2C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPAP2C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | Available on request — contact us for lot-specific performance data |
| Sensitivity | Available on request — contact us for lot-specific performance data |
| Species Reactivity | Bovine (Bos taurus; Cattle) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE60069BO |
| UniProt # | Q2HJ61 |
| Alternative Names | LPP2; PAP-2c; PAP2-g; lipid phosphate phosphohydrolase 2|phosphatidic acid phosphohydrolase type 2c|type-2 phosphatidic acid phosphatase-gamma |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Bovine ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for PPAP2C research.
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