| Field | Specification |
|---|---|
| Target | |
| Alternative names | DKFZp586I0923; Hqk; QK; QK1; QK3; RNA binding protein HQK|homolog of mouse quaking QKI (KH domain RNA binding protein)|quaking homolog; KH domain RNA binding |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
QKI belongs to a family of RNA-binding proteins called STAR proteins for Signal Transduction and Activation of RNA. They have an HNRNPK homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1) and SF1. The QKI gene is implicated as being important in schizophrenia, and QKI controls translation of many oligodendrocyte-related genes.The balance between the nuclear and cytoplasmic isoforms of Qk1 controlled the nuclear export of Mbp mRNAs and the cellular localization of the 17- and 21.5-kD Mbp isoforms. Overexpression of Qki-5 in cultured rodent oligodendrocytes recreated the Mbp defects observed in qk(v) mice, with retention of Mbp in the nucleus.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate QKI in samples. An antibody specific for QKI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyQKI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QKI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QKI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | Available on request — contact us for lot-specific performance data |
| Sensitivity | Available on request — contact us for lot-specific performance data |
| Species Reactivity | Bovine (Bos taurus; Cattle) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE24879BO |
| UniProt # | Q5W9D7 |
| Alternative Names | DKFZp586I0923; Hqk; QK; QK1; QK3; RNA binding protein HQK|homolog of mouse quaking QKI (KH domain RNA binding protein)|quaking homolog; KH domain RNA binding |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Bovine ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for QKI research.
Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
