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This kit uses either BCA (bicinchoninic acid) or Bradford (Coomassie G-250) colorimetric chemistry. BCA is more detergent-compatible and provides a broader linear range, while Bradford is faster and less sensitive to reducing agents. The choice depends on your sample matrix — refer to the specific product datasheet for the validated linear range, compatibility, and recommended standard curve options.
This kit includes BSA (bovine serum albumin) as the protein standard. BSA is the most commonly used reference protein; however, if your target protein has a significantly different amino acid composition or extinction coefficient from BSA, relative quantification results may vary. For absolute quantification of a specific protein, use a calibrated standard of that protein. The kit datasheet specifies the standard concentration range and dilution series.
BCA is compatible with most non-ionic detergents (Triton X-100, Tween-20, NP-40) at low concentrations but is inhibited by strong reducing agents (DTT, β-mercaptoethanol) and EDTA above certain thresholds. Bradford is sensitive to detergents including SDS (>0.1%) and Triton X-100. Match your lysis buffer components to the method's compatibility profile — refer to the datasheet interference table for validated concentration limits.
Yes. This kit is formatted for standard 96-well microplate readers with absorbance measurement at 562 nm (BCA) or 595 nm (Bradford). The Ready-to-Use format further simplifies setup — reagents are pre-mixed and dispensed directly to the sample without additional preparation. Ensure your plate reader has been calibrated and the wavelength matches the assay format.
Yeasen Biotechnology offers flexible customization options for many of its assay kits and detection reagents, including custom lot sizes, bulk ordering, and application-specific formulation adjustments. Volume pricing, custom packaging, and kit bundling may be available depending on the product and intended workflow. A Certificate of Analysis (CoA) and lot-specific QC data are provided with every order. For inquiries regarding large-volume orders, custom configurations, or integration into automated workflows, please contact the BioHippo team for a tailored quotation.
