| Field | Specification |
|---|---|
| Species |
The BRL-3A cell line is derived from the normal liver of a male Buffalo rat. Established in 1976, this cell line is an important in vitro model primarily used for studying hepatocyte function, liver regeneration mechanisms, and hepatotoxicity. BRL-3A cells retain several characteristics of primary hepatocytes, including the ability to synthesize albumin and other serum proteins, making them a valuable tool in hepatological research. These cells exhibit an epithelial-like morphology and are adherent with a high growth rate in culture.
Scientific interest in BRL-3A extends to its application in the study of liver-specific viral infections, drug metabolism, and the effects of various growth factors and cytokines on liver cells. Researchers also utilize BRL-3A cells to investigate the impact of toxins and carcinogens on liver function, providing insights into hepatocarcinogenesis and liver injury. The cells are known to respond to peroxisome proliferators and have been used to test the efficacy and safety of pharmaceuticals potentially affecting liver function.
However, despite their versatility, users of the BRL-3A cell line must consider the limitations inherent to a non-human model, as results may not always directly translate to human liver physiology. This factor underscores the importance of corroborating findings with additional models and experimental approaches.
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Products: Multiplication stimulating activity (MSA).
- cultureMedium: Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: Seeding density of 1 x 104 cells/cm2 is recommended.
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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