| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, Hygromycin, Puromycin, Zeocin |
| Shipping | |
| Species |
Background
BRN3A (POU4F1) and BRN3B (POU4F2) are class IV POU-domain transcription factors that bind DNA through a bipartite POU domain. Categorized as non-octamer-binding proteins because of their reduced affinity for canonical octamer motifs, they instead recognize distinct BRN3 elements such as those found in the corticotropin-releasing hormone promoter. BRN3A is expressed in retinal ganglion cells, peripheral sensory neurons, and lymphoid lineages, where it directs sensory neuron differentiation and survival, retinal ganglion cell development, and aspects of immune function. BRN3B is similarly important for retinal ganglion cell specification and axon guidance. These factors are studied as regulators of neuronal development and visual system biology and are of interest in neuroscience and developmental research.
Product Description & Applications
The BRN3 Reporter Lentivirus is a transcription factor reporter system that detects transcriptional activity mediated by BRN3A and BRN3B in mammalian cells. The construct contains tandem repeats of BRN3 DNA-binding elements derived from the rat CRH promoter, placed upstream of a minimal promoter to drive a fluorescent or luminescent reporter. An optimized upstream enhancer maximizes signal-to-noise, and a constitutive drug selection marker (Blasticidin, Hygromycin, Puromycin, or Zeocin) enables generation of stable polyclonal reporter cell lines. Stable lentiviral integration provides consistent reporter expression suitable for readout by fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is well suited to studying BRN3 activity in primary and difficult-to-transfect cells.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the BRN3 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
