BS-C-1 cell

SKU:BHC11101254
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Overview
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BS-C-1 cell is a cell line. It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Epithelial. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Chlorocebus pygerythrus (Vervet monkey)
Morphology Epithelial
Growth Properties Adherent
Tissue Kidney
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Catalog no. Size
305009 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305009
Species Chlorocebus pygerythrus (Vervet monkey)
The BS-C-1 cell line, also known as Cercopithecus aethiops kidney cells, originates from the kidney of the African green monkey. This cell line, established in the 1960s, is used extensively in virology research due to its susceptibility to adenoviruses, simian viruses, and other pathogenic agents. BS-C-1 cells exhibit epithelial morphology and are adherent in culture, making them suitable for a variety of experimental setups, including virus-host interaction studies and cytotoxicity assays. One of the distinguishing features of BS-C-1 cells is their utility in the propagation and maintenance of polioviruses, which facilitates vaccine development and virus lifecycle studies. The cells are also known for their role in the discovery and study of adenoviruses, contributing significantly to our understanding of viral genetics and replication processes. Despite their origins and primary uses, BS-C-1 cells have also been employed in pharmacological research and toxicology, testing the effects of various substances on cellular processes and viability. Due to their robust growth characteristics and ability to be transfected relatively easily, BS-C-1 cells are valuable in molecular biology for gene expression studies. Their compatibility with a wide range of DNA transfection methods supports their use in gene therapy research and recombinant protein production. Overall, BS-C-1 cells continue to be a critical resource in biomedical research, providing insights into cellular behavior and the molecular basis of disease.

SKU:BHC11101254

Protein expression: Keratin

  • cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
  • supplements: Supplement the medium with 10% FBS and 1% NEAA
  • dissociationReagent: Accutase
  • doublingTime: 72 hours
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • fluidRenewal: 2 to 3 times per week
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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