| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
This product is a type II restriction endonuclease, a recombinant protein encoded by the BspQI gene from Bacillus sphaericus expressed in E. coli. It recognizes the sequence 5'-GCTCTTCN1/N4-3' and is mainly used for digesting plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends, obtaining specific sticky ends.
Applications
Digesting plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends.
Digesting DNA to obtain specific sticky ends.
Specifications
|
Recognition site |
5'-GCTCTTC(N)↓-3' 3'-CGAGAAG(N)4↑-5' |
|
Concentration |
10 U/µL |
|
Definition of Unit |
The amount of enzyme required to digest 1 µg of λDNA in a 50 µL reaction volume at 50°C within 1 hour is defined as one unit (U) of activity. |
|
Reaction conditions |
React at 50℃ for 60 min |
Glycerol Content |
Contains Glycerol |
Components
|
Name |
15202ES76 |
15202ES86 |
15202ES92 |
|
BspQI (10 U/μL) |
50 μL |
250 μL |
1 mL |
Shipping and Storage
The products should be stored at -25℃ ~ -15℃ for 1 year.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Digesting plasmids to prepare linearized DNA fragments with poly(A/T/G/C) ends. Digesting DNA to obtain specific sticky ends.. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme required to completely digest 1 µg of lambda DNA (or specified substrate DNA) in 60 min at the recommended incubation temperature in a total reaction volume of 50 µL.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Restriction enzyme efficiency depends on buffer composition (NaCl/KCl concentration, pH, Mg²⁺), incubation temperature (typically 37°C), and template quality. Star activity—non-specific cleavage at relaxed recognition sequences—can occur under conditions of excess enzyme, extended incubation, high glycerol concentration, or non-optimal ionic strength. Use of manufacturer-supplied buffers and adherence to recommended enzyme:DNA ratios (typically 1–10 U per µg DNA) minimizes star activity.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
