| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP dependent helicase BTAF1; ATP-dependent helicase BTAF1; B-TFIID transcription factor associated 170 kDa subunit; B-TFIID transcription factor associated, 170kDa (Mot1 homolog, S. cerevisiae); B-TFIID transcription factor-associated 170 kDa subunit; BTAF 1 RNA polymerase II; BTAF1; BTAF1 RNA polymerase II B TFIID transcription factor associated 170kDa; BTAF1_HUMAN; MOT 1; MOT1; MOT1 Yeast homolog of; TAF 172; TAF II 170; TAF(II)170; TAF-172; TAF172; TAFII170; TATA binding protein associated factor 172; TATA-binding protein-associated factor 172; TBP associated factor 172; TBP-associated factor 172 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human TATA-binding protein-associated factor 172 protein (1750-1849AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-BTAF1 antibody raised in Rabbit, conjugated with Biotin, tested in ELISA. Designed to detect BTAF1 in Human for epigenetics and nuclear signaling research.
Key elements and design rationale
- Immunogen: Recombinant Human TATA-binding protein-associated factor 172 protein (1750-1849AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- Biotin: Streptavidin/avidin amplification; block endogenous biotin in biotin-rich samples.
Biological background
BTAF1 (also: ATP dependent helicase BTAF1, ATP-dependent helicase BTAF1, B-TFIID transcription factor associated 170 kDa subunit, B-TFIID transcription factor associated, 170kDa (Mot1 homolog, S. cerevisiae), B-TFIID transcription factor-associated 170 kDa subunit, BTAF 1 RNA polymerase II, BTAF1, BTAF1 RNA polymerase II B TFIID transcription factor associated 170kDa, BTAF1_HUMAN, MOT 1, MOT1, MOT1 Yeast homolog of, TAF 172, TAF II 170, TAF(II)170, TAF-172, TAF172, TAFII170, TATA binding protein associated factor 172, TATA-binding protein-associated factor 172, TBP associated factor 172, TBP-associated factor 172) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current BTAF1 annotation relevant to epigenetics and nuclear signaling.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
BTAF1 is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects BTAF1 in ELISA applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Biotin: streptavidin/avidin amplification. Block endogenous biotin in biotin-rich samples.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing BTAF1; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
