C3 Antibody, HRP conjugated

Overview

This polyclonal anti-C3 antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect C3 in Rat for immunology research.

Key elements and design rationale

• Immunogen: Recombinant Rat Complement C3 protein (671-746AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
• HRP: Direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.

Biological background

C3 (also: C3Complement C3 [Cleaved into: Complement C3 beta chain, C3-beta-c, C3bc, Neutrophil chemotactic factor-2, ENCF-2), Complement C3 alpha chain, C3a anaphylatoxin, Neutrophil chemotactic factor-1, ENCF-1), Acylation stimulating protein, ASP, C3adesArg), Complement C3b alpha' chain, Complement C3c alpha' chain fragment 1, Complement C3dg fragment, Complement C3g fragment, Complement C3d fragment, Complement C3f fragment, Complement C3c alpha' chain fragment 2]) — studied in Rattus norvegicus (Rat) systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current C3 annotation relevant to immunology.

Common research applications

• ELISA: Quantify soluble target; ensure samples within the linear detection range.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Rat; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12554219)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.