| Field | Specification |
|---|---|
| Target | |
| Alternative names | Interleukin-4; IL-4; B-cell stimulatory factor 1; BSF-1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; IL4 |
| Gene ID | |
| UniProt # | |
| Reactivity | |
| Applications | |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: His25-His132 |
| Assay type | |
| Sample type(s) | Cell Culture Supernatant, Serum, Plasma |
| Sensitivity | |
| Detection range | |
| Assay time | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Assay Principle
This sandwich ELISA quantifies canine interleukin-4 (gene IL4) in cell culture supernatants, serum and plasma (heparin, EDTA). Strips are pre-coated with a capture antibody. After the sample is added, a biotinylated detection antibody binds the captured protein, and an avidin–biotin–peroxidase complex supplies the enzyme. TMB develops color in proportion to the bound target; the reaction is stopped and read at 450 nm, and concentrations are read from the standard curve.
Interleukin-4 (IL-4) is a key type 2 cytokine made by Th2 cells, mast cells, basophils and eosinophils. Signaling through IL-4Rα paired with either the common gamma chain or IL-13Rα1, it activates JAK–STAT6 to drive Th2 differentiation, B-cell class switching to IgE and alternative macrophage activation. It is a core readout in allergy and parasite immunity studies.
Kit Components
| Anti-Canine IL4 Pre-coated 96-well Strip Microplate | 12 strips of 8 wells |
|---|---|
| Canine IL4 Standard | 2 × 10 ng/tube |
| Canine IL4 Biotinylated Antibody (100x) | 100 µL |
| Avidin-Biotin-Peroxidase Complex (100x) | 100 µL |
| Sample Diluent | 30 mL |
| Antibody Diluent | 12 mL |
| Avidin-Biotin-Peroxidase Diluent | 12 mL |
| Color Developing Reagent (TMB) | 10 mL |
| Stop Solution | 10 mL |
| Wash Buffer (25x) | 20 mL |
| Plate Sealers | 4 |
Also required (not supplied): microplate reader for 450 nm; incubator; automated plate washer (optional); precision pipettes for 0.5 µL through 1 mL volumes of aqueous solutions; multichannel pipettes (recommended for many samples); deionized or distilled water; 500 mL graduated cylinders; test tubes for dilution.
Performance Data
| Species | Canine |
|---|---|
| Sample types | Cell culture supernatants, serum, plasma (heparin, EDTA) |
| Assay range | 93.8–6,000 pg/mL |
| Sensitivity | <15 pg/mL |
| Standard | Recombinant canine IL4 (His25–His132), expressed in E. coli |
| Cross-reactivity | No significant cross-reactivity or interference with IL4 analogs in supplier testing; untested analogs cannot be excluded. |
Storage & Handling
Store at 4 °C for up to 6 months or −20 °C for up to 12 months. Avoid repeated freeze–thaw cycles. Ships on gel ice and tolerates up to 3 days at room temperature; freeze on arrival.
Safety
Sample data
| Concentration (pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.01 | 0.049 | 0.118 | 0.218 | 0.442 | 0.795 | 1.289 | 1.882 |
Intra/inter assay consistency
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 129 | 451 | 1884 | 162 | 494 | 1931 |
| Standard deviation | 6.90 | 27.01 | 72.16 | 5.38 | 38.88 | 56.58 |
| CV (%) | 5.4% | 6.0% | 3.8% | 3.3% | 7.9% | 2.9% |
Kit components
Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4Can’t Find What You’re Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
