| Field | Specification |
|---|---|
| Target | |
| Alternative names | MGC116783; MGC116785; TP2; |
| UniProt # | |
| Species | |
| Applications | |
| Assay type | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
TNP2 were not found in human testicular RNA or human testis cDNA libraries. Finding homologous sequences in the human genome was greatly facilitated, however, by the finding that, in the boar and bull genomes, the genes for protamine-1 (PRM1) and Prm2 are clustered with Tnp2. Schluter et al. (1992) tested a genomic human cosmid clone containing the genes for both protamines and found that the TNP2 gene was within a distance of 7.5 kb 3-prime of the PRM2 gene. The gene was transcribed in human testicular RNA only at a level demonstrable by RT-PCR and RNase protection assay. In contrast to the TNP2 genes of mouse, rat, boar, and bull, the human TNP2 gene lacks the nucleotide sequence GCCATCAC in its 3-prime UTR.
Assay Principle
This assay employs a two-site sandwich ELISA to quantitate TNP2 in samples. An antibody specific for TNP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Performance Data
| Assay Type | Sandwich ELISA |
|---|---|
| Detection Range | Available on request — contact us for lot-specific performance data |
| Sensitivity | Available on request — contact us for lot-specific performance data |
| Species Reactivity | Canine (Canis familiaris; Dog) |
| Stability | The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C). |
| Manufacturer No. | AE13885DO |
| UniProt # | O77645 |
| Alternative Names | MGC116783; MGC116785; TP2; |
Assay Workflow
The kit includes the components required for standard-curve quantification (see the kit manual for the component table and validated sample types). Absorbance is read on a standard microplate reader and concentrations are interpolated from the standard curve.
Safety & Handling
For research use only (RUO). Not for use in diagnostic or therapeutic procedures. Handle all samples and reagents using standard laboratory safety practices.
Related Products
Explore additional Canine ELISA kits from Wuhan Abebio Science Co Ltd, or contact us for related assays for TNP2 research.
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