| Field | Specification |
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| Form | Liquid |
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Product Overview
Carboxyl Iron Oxide Nanoparticles Conjugation Kit is a Conjugation Kit from Ocean NanoTech.
Ocean NanoTech’s carboxyl terminated magnetic iron oxide nanoparticles (SHPs) of various diameters (5 nm-30 nm) are available in an easy-to-use kit format to enable researchers to conjugate antibodies, proteins, peptides or other biomolecules of their own choice to these magnetic iron oxide nanoparticles. The kit contains sufficient reagents and components for performing 5 conjugation reactions using 1 mg magnetic iron oxide nanoparticles per reaction. Briefly, the magnetic iron oxide nanoparticles are conjugated to amine groups that are present on the target protein/ligand using carbodiimide. The protocol shown below has been used to successfully conjugate bovine serum albumin, streptavidin, and immunoglobulin to Ocean Nanotech’s magnetic iron oxide nanoparticles.
Kit Contents
- Carboxyl Iron Oxide Nanoparticles: 1 mL
- Activation Buffer (AB300): 10 mL
- Quenching Buffer (QB100): 1 mL
- Storage Buffer (SB300): 15 mL
- EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide), 20 mg (Store at -20 °C upon arrival)
Physical & Chemical Properties
| pH | 6.0 - 8.0 at 25 °C (77 °F) |
|---|---|
| Solubility | Completely miscible |
| Incompatible Materials | Strong oxidizing agents |
Safety & Handling
For Research Use Only (RUO). Consult the Safety Data Sheet (SDS) before use and follow all applicable safety protocols.
This conjugation kit is optimized for covalent attachment of proteins, antibodies, peptides, and other amine- or carboxyl-containing biomolecules to the nanoparticle surface. The conjugation chemistry exploits EDC/NHS carbodiimide coupling or direct amine coupling depending on the specific kit protocol. Refer to the product data sheet for the list of validated biomolecule types and loading ratios.
This kit is available in multiple particle size variants (accessible via the variant selector). Particle size affects hydrodynamic behavior, conjugation efficiency, and downstream assay sensitivity. Smaller particles (10–15 nm) are preferred for solution-phase immunoassays, while larger particles (20–30 nm) may be better suited for lateral flow or magnetic separation applications.
Conjugation efficiency can be assessed by measuring protein concentration in the pre- and post-conjugation supernatant using a BCA or Bradford assay to estimate the amount of protein immobilized on the nanoparticle surface. Gel electrophoresis, DLS (dynamic light scattering), and functional assays (e.g., antibody binding activity) are recommended for comprehensive conjugate characterization.
Conjugated nanoparticles should be stored in the supplied storage buffer at 2–8°C and protected from light. Avoid freezing, as freeze-thaw cycles can cause irreversible aggregation. Short-term storage at room temperature is acceptable for applications requiring immediate use, but long-term stability should be evaluated under intended storage conditions.
BioHippo partners with leading life science suppliers to offer flexible purchasing and support options. Contact our team to discuss the following add-on services.
- Bulk & Volume Pricing: Reduced per-unit pricing for multi-unit or project-scale orders. Submit a quote request for a customized offer.
- Expedited Fulfillment: Priority order handling may be available for time-sensitive projects. Confirm availability before ordering.
- Technical Consultation: Our scientific team provides protocol guidance and troubleshooting at no additional charge.
- Custom Configurations: Alternative formulations, pack sizes, or kit configurations may be available upon request.
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