Carboxylated Plates

Carboxylated Plates are ideal for promoting the covalent immobilization of compounds with reactive free amino groups. The surface features a negatively charged carboxylated layer, enabling the binding of peptides and other molecules through specific covalent bonds using EDC-mediated amination. This method overcomes several limitations, including the immobilization of small molecules (1,000–5,000 Da), such as peptides, drugs, toxins, or hormones, which may otherwise bind weakly or not at all through physical adsorption. Additionally, it ensures oriented immobilization, maintaining the integrity and accessibility of specific sites, and offers increased storage stability by minimizing the risk

Carboxyl Treated 96 Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Key Features

• Enhanced binding efficiency for small molecules like peptides (1,000–5,000 Da), drugs, toxins, and hormones, which typically struggle with physical adsorption.
• Oriented immobilization, ensuring that molecules retain their structural integrity and maintain accessible active sites for optimal functionality.
• Improved storage stability, significantly reducing the risk of spontaneous desorption, leading to more reliable and long-lasting immobilization compared to physical adsorption methods.
• Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
• Wells design: flat bottom.
• Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
• Colors: Available in clear, white, and black. Customizable upper rim colors upon request. Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays. Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
  • Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
  • Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
• Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
• Alphanumeric Coding: For easy and accurate well identification during experiments.
• Recommended working volume: of 75 to 200 μl
• Packaging: 5 plates in a transparent bag.
• Unit: Contains 5 plates
• Minimum order: 10 plates.
• Ready to use

Key Benefits

• Plates Design: The mould design provides optical quality, important to reduce the background signal. The rim protects the external face of the bottom from scratches
  • The mould design provides optical quality, important to reduce the background signal.
  • The rim protects the external face of the bottom from scratches
• Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
• Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
• Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
• Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Applications

• ELISA Assays: For competitive and sandwich ELISA, detecting a wide range of targets such as steroid hormones and antibodies.
• Luminescence Assays (LIA): Optimized for high-sensitivity luminescence detection.
• Fluorescence Assays (FIA): Ensures strong signal detection with minimal interference.
• Chemiluminescent Assays (CLIA): Ideal for highly sensitive and quantitative analyses.