| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C13H17ClN4O2 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
Cardiogenol C hydrochloride is a potent, cell-permeable pyrimidine inducer that prompts the differentiation of embryonic stem cells (ESCs) into cardiomyocytes, with an EC50 of 100 nM[1]. It also acts cardiomyogenically on already lineage-committed progenitor cell types with a limited degree of plasticity, and it can be used as a tool to improve cardiac repair by cell transplantation therapy in animal models[2]. It is supplied as a white to off-white solid (C13H17ClN4O2, MW 296.75) at 99.79% purity.
Physical & Chemical Properties
| CAS Number | 1049741-55-0 |
|---|---|
| Molecular Formula | C13H17ClN4O2 |
| Molecular Weight | 296.75 g/mol |
| Purity | 99.79% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | [H]Cl.COC1=CC=C(NC2=NC=CC(NCCO)=N2)C=C1 |
| Signaling Pathway | Stem Cell/Wnt |
| Solubility | In Vitro: DMSO: ≥ 59 mg/mL (198.82 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) H2O: 2 mg/mL (6.74 mM; Requires sonication) * "≥" means soluble, but saturation unknown. |
| Storage | 4°C, sealed storage, away from moisture. In solvent: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Biological Activity
Activity & Target
EC50: 100 nM (differentiation of ESCs into cardiomyocytes)[1]
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | ≥ 59 mg/mL (198.82 mM) | use freshly opened DMSO (absorbed moisture lowers solubility) |
| H2O | 2 mg/mL (6.74 mM) | requires sonication |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); sealed storage, away from moisture; avoid repeated freeze-thaw cycles.
If water is used as the stock solvent, dilute to the working solution and sterilize it through a 0.22 μm filter before use.
In Vivo
Choose the formulation that suits the animal model and route of administration; percentages are volume ratios of the final working solution. Start from a clear DMSO stock (see In Vitro above), add the co-solvents one at a time in the order listed, mixing after each addition, and prepare the working solution fresh on the day of dosing. If precipitation or phase separation occurs, gentle warming or sonication can help.
Protocol 1
| Composition | 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% saline |
|---|---|
| Result | 2.5 mg/mL (8.42 mM); clear solution; requires sonication |
| How to prepare | Gives a clear solution at 2.5 mg/mL. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 400 μL PEG300; then 50 μL Tween-80; then 450 μL saline to bring the volume to 1 mL. Saline: dissolve 0.9 g sodium chloride in ddH2O and make up to 100 mL. |
Protocol 2
| Composition | 10% DMSO + 90% (20% SBE-β-CD in saline) |
|---|---|
| Result | ≥ 2.5 mg/mL (8.42 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL 20% SBE-β-CD in saline. 20% SBE-β-CD in saline: dissolve 2 g SBE-β-CD powder in 10 mL saline until clear (4°C, store up to one week). |
Protocol 3
| Composition | 10% DMSO + 90% Corn Oil |
|---|---|
| Result | ≥ 2.5 mg/mL (8.42 mM); clear solution |
| How to prepare | Gives a clear solution at ≥ 2.5 mg/mL (saturation not determined). Use with caution if continuous dosing will exceed two weeks. For 1 mL of working solution: add 100 μL DMSO stock (25.0 mg/mL) to 900 μL corn oil. |
Direct preparation of the working solution
These formulations are prepared directly, without a DMSO stock; use them promptly after preparation.
Protocol 4
| Composition | PBS |
|---|---|
| Result | 3.33 mg/mL (11.22 mM); clear solution; requires sonication |
Data provided by the manufacturer.
In Vitro
Cardiogenol C hydrochloride (1 μM; 7 days) exerts a cardiomyogenic effect on P19 cells, significantly raising atrial natriuretic factor (ANF, nppa) in P19 cells compared with untreated control cells[1]. Cardiogenol C hydrochloride (0.01-100 μM; 7 days) significantly raises ANF expression. Another widely used cardiac marker gene (NKX2-5) is also significantly raised by this small molecule in C2C12 cells[2]. Cardiogenol C hydrochloride (0.001-100 μM; 7 days) increases expression of the cardiac Nav1.5 sodium channel protein in a dose-dependent manner in C2C12 cells[2]. When Cardiogenol C hydrochloride (1 μM; 35 days) is added from day 0, myocardial differentiation increases significantly and the percentage of CBs with beating cardiomyocytes rises significantly. This small molecule promotes development of beating cardiomyocytes in cardiac bodies derived from cardiovascular progenitor cells[2]. Cardiogenol C hydrochloride (0.01-100 μM; 7 days) has no effect on cell growth, even at 10 μM. Cardiogenol C dissolved in either water or DMSO gives a similar effect. The highest concentration, 100 μM, shows significant cellular toxicity in C2C12 cells[2].
RT-PCR[2]
| Cell Line | C2C12 cells |
|---|---|
| Concentration | 0.01 μM; 0.1 μM; 1 μM; 3 μM; 10 μM; 30 μM; 100 μM |
| Incubation Time | 7 days |
| Result | Increased ANF and NKX2.5 mRNA level as a dose-dependent manner. |
Western Blot Analysis[2]
| Cell Line | C2C12 cells |
|---|---|
| Concentration | 0.001 μM; 0.01 μM; 0.1 μM; 1 μM; 10 μM |
| Incubation Time | 7 days |
| Result | Increased cardiac Nav1.5 sodium channel protein levels. |
Cell Proliferation Assay[2]
| Cell Line | C2C12 cells |
|---|---|
| Concentration | 0.01 μM; 0.1 μM; 1 μM |
| Incubation Time | 7 days |
| Result | Did not exert toxic effects on C2C12 cells at 0.01-10 μM treatment. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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