| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Reporter | |
| Selection Marker | Hygromycin, Zeocin, Puromycin, Blasticidin |
| Shipping | |
| Species |
Background
CAR (constitutive androstane receptor, NR1I3) is a ligand-activated transcription factor of the nuclear receptor superfamily and a master regulator of xenobiotic and drug metabolism. CAR heterodimerizes with the retinoid X receptor alpha (RXRα) and binds CAR response elements (CARE), often arranged as direct repeats, in the promoters of cytochrome P450 and other detoxification genes. CAR can be activated through both direct ligand binding and ligand-independent mechanisms, and the CAR-RXRα heterodimer participates in drug metabolism, energy homeostasis, and the modulation of cancer development by regulating numerous target genes, predominantly in hepatocytes. These functions make CARE-driven transcription an informative readout in metabolism and pharmacology research.
Product Description & Applications
The CARE Reporter Lentivirus is a transcription-factor reporter system engineered to detect the transcriptional activity of the constitutive androstane receptor in human and mouse cells. The construct uses tandem repeats of CAR response elements derived from the cytochrome P450 (CYP2B10) gene promoter, arranged in a DR4 pattern to read out the synergistic activity of the CAR/RXRα heterodimer characteristic of hepatocytes. A fluorescent or luminescent reporter is paired with a constitutively expressed selection marker for stable polyclonal cell line generation.
Readout is by microscopy, flow cytometry, or luminometry. Applications include studying CAR-mediated drug metabolism, energy homeostasis, and CAR pharmacology. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.
About This Product
This reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the CAR1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Hygromycin, Zeocin, Puromycin, Blasticidin) and/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.
Stable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
