Cas9-Expressing HEK293 Cell Line

What it means: The parental cell line is the host in which the transgene or reporter was stably integrated. HEK293 (Human Embryonic Kidney 293) is a epithelial-like, adherent cell line selected for this product.

Why it matters:

• Enables high transfection efficiency and robust transgene expression; widely used for receptor pharmacology, ion channels, and reporter gene assays.
• The parental background determines endogenous receptor expression, growth properties, and media requirements—verify compatibility with your assay conditions.
• Secondary reagents (antibodies, growth media) should be validated for this host species and cell type.

How to interpret here: This product is engineered on a HEK293 background. Pathway activity readouts, IC50/EC50 values, and control responses are calibrated for this host. Results may differ in alternative cell backgrounds.

What it means: Species refers to the biological origin of the expressed gene or protein target in this cell line. This product is classified as S. pyogenes origin.

Why it matters:

• This product is of S. pyogenes origin. Confirm reagent compatibility for your specific experimental context.
• Species origin directly affects antibody cross-reactivity, functional assay interpretation, and translational relevance of results.
• Confirm that your detection antibodies, assay controls, and signaling reagents are validated for S. pyogenes targets.

How to interpret here: The S. pyogenes designation indicates the sequence and biological context of the expressed construct—not necessarily the host cell species. Check the host cell line chip for the cellular background.

What it means: BSL-2 (Biosafety Level 2) indicates this material poses moderate risk and requires specific containment practices and training.

Why it matters:

• Work must be performed in a certified BSL-2 laboratory; access is restricted to trained personnel.
• All procedures generating aerosols or splashes must be conducted in a Class II Biological Safety Cabinet (BSC).
• PPE required: lab coat, gloves, and eye/face protection. Decontaminate all waste before disposal.
• Institutional Biosafety Committee (IBC) approval is required prior to use.

How to interpret here: BSL-2 classification means your facility must be BSL-2 certified and your team trained for BSL-2 handling before working with this product. Confirm current IBC approval status and maintain a decontamination log.

Tip: If you are uncertain whether your laboratory is BSL-2 certified, contact your institutional Environmental Health & Safety (EH&S) office.

What it means: CRISPR KO Cell Line. A CRISPR KO Cell Line has the target gene disrupted using CRISPR/Cas9 genome editing to create a loss-of-function model validated at the genomic and protein level.

Why it matters:

• Provides an isogenic null background for mechanistic studies, target validation, and off-target screening without confounding effects of overexpression systems.
• Select the correct product type based on whether your experiment requires overexpression, loss-of-function, pathway reporting, or immune effector function.

How to interpret here: This CRISPR KO Cell Line eliminates endogenous expression of the indicated target. Confirm knockout by Western blot and genomic sequencing in your lab before experimental use.

What it means: Confirmed Negative indicates this cell line was screened using a validated mycoplasma detection assay prior to release and confirmed negative for mycoplasma contamination.

Why it matters:

• Mycoplasma contamination can significantly alter gene expression profiles, proliferation rates, metabolism, and assay readouts—potentially invalidating experimental results without visible signs of contamination.
• Testing prior to use is considered best practice in accordance with ATCC and ECACC guidelines for cell line authentication.
• BPS Bioscience uses validated PCR-based or luminescence-based detection kits (e.g., Venor™GeM, MycoAlert™) for screening.

How to interpret here: Confirmed Negative confirms the product shipped to you passed mycoplasma screening at the time of manufacture. We still recommend performing routine mycoplasma testing in your laboratory after receipt and during extended culture to confirm ongoing negative status.

Tip: Test your cell cultures for mycoplasma at regular intervals (e.g., every 2–4 weeks) regardless of the vendor QC status. Contamination can be introduced during routine culture handling.

What it means: Liquid Nitrogen storage indicates this cell line must be maintained in vapor-phase or liquid-phase liquid nitrogen (≤-130°C) for long-term preservation of viability and function.

Why it matters:

• Liquid nitrogen cryopreservation halts cellular metabolism and ice crystal formation, preserving cell viability and transgene expression stability for extended periods.
• Storage above -130°C (e.g., -80°C freezer) is not equivalent—cells may lose viability within weeks to months at -80°C.
• Transfer cells from dry-ice shipping to Liquid Nitrogen storage immediately upon receipt to prevent viability loss.

How to interpret here: Liquid Nitrogen storage is required for this product. Access to a liquid nitrogen Dewar or cryogenic storage tank is essential before ordering. Do not store in a standard -80°C freezer as long-term cryostorage.