CASP3 Antibody

SKU:BHA10543524
Overview
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CASP3-specific polyclonal antibody raised in Rabbit. Validated for Immunohistochemistry, ELISA in Human, Mouse, Rat samples. Affinity-purified.
Format Unconjugated
Immunogen Native Protein
Applications 2 validated
Dilution IHC:1:50-1:300, ELISA:1:5000-1:20000
Target Aliases 38 aliases
Options selector
Catalog no. Size
CSB-PA140280-50UG 50 ug
CSB-PA140280-100UG 100 ug
Available Options
Size
Field Specification
Target CASP3
Antigen
  • Synthetic peptide from Human protein at AA range: 1268-1341
Alternative names A830040C14Rik; Apopain; CASP 3; CASP-3; CASP3; CASP3_HUMAN; Casp3a; Caspase 3; Caspase 3; apoptosis-related cysteine peptidase; Caspase 3; apoptosis-related cysteine protease; Caspase 3; apoptosis-related cysteine protease a; Caspase-3 subunit p12; Caspase3; CC3; CPP 32; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; ICE3; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; Protein Yama; SCA 1; SCA-1; SCA1; SREBP cleavage activity 1; Yama; Yama protein
UniProt # P42574
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Immunohistochemistry
  • ELISA
Immunogen Synthetic peptide from Human protein at AA range: 1268-1341
Conjugate
  • Non-conjugated
Source This product is a polyclonal antibody purified from rabbit antiserum.
Purification Antigen affinity purification
Form Liquid
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage buffer 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Catalog no. (Mfr.) CSB-PA140280
Main SKU BHA10543524

Product Overview

Primary Antibody

This antibody targets CASP3 and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
Immunohistochemistry (IHC) IHC:1:50-1:300, ELISA:1:5000-1:20000
ELISA IHC:1:50-1:300, ELISA:1:5000-1:20000
✔ Validated by CUSABIO Technology

Antibody Specifications

Target CASP3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Purification Affinity Purification
Antigen / Immunogen Synthetic peptide from Human protein at AA range: 1268-1341
Production This product is a polyclonal antibody purified from rabbit antiserum.
Form Liquid
Storage Buffer 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names

A830040C14Rik; Apopain; CASP 3; CASP-3; CASP3; CASP3_HUMAN; Casp3a; Caspase 3; Caspase 3; apoptosis-related cysteine peptidase; Caspase 3; apoptosis-related cysteine protease; Caspase 3; apoptosis-related cysteine protease a; Caspase-3 subunit p12; Caspase3; CC3; CPP 32; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; ICE3; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; Protein Yama; SCA 1; SCA-1; SCA1; SREBP cleavage activity 1; Yama; Yama protein

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetCASP3
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ConjugateUnconjugated
ReactivityHuman, Mouse, Rat
PurificationAffinity Purification
Antigen/ImmunogenSynthetic peptide from Human protein at AA range: 1268-1341
ProductionThis product is a polyclonal antibody purified from rabbit antiserum.
FormLiquid
Storage Buffer0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
Immunohistochemistry (IHC)IHC:1:50-1:300, ELISA:1:5000-1:20000
ELISAIHC:1:50-1:300, ELISA:1:5000-1:20000

Immunogen: Synthetic peptide from Human protein at AA range: 1268-1341

Antigen Species: Human

Alternative Names

A830040C14Rik; Apopain; CASP 3; CASP-3; CASP3; CASP3_HUMAN; Casp3a; Caspase 3; Caspase 3; apoptosis-related cysteine peptidase; Caspase 3; apoptosis-related cysteine protease; Caspase 3; apoptosis-related cysteine protease a; Caspase-3 subunit p12; Caspase3; CC3; CPP 32; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; ICE3; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; Protein Yama; SCA 1; SCA-1; SCA1; SREBP cleavage activity 1; Yama; Yama protein

What species does this CASP3 antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: Immunohistochemistry (IHC), ELISA. Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
IHC:1:50-1:300, ELISA:1:5000-1:20000. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Rabbit and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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