| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17, Caspase-3 subunit p12], CASP3, CPP32 |
| Antigen | |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | A synthesized peptide derived from human pro Caspase 3 |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a recombinant monoclonal antibody expressed in HEK293F cells. |
| Storage | |
| Target | |
| UniProt # |
Product Overview
Research Use OnlyCASP3 is a research-grade antigen target for which this antibody provides selective detection in commonly studied model systems.
Validated Applications
| ELISA (ELISA) | See datasheet for recommended dilution |
|---|---|
| Western Blot (WB) | 1:500-1:5000 |
| Immunohistochemistry (IHC) | 1:50-1:200 |
| Immunofluorescence (IF/ICC) | See datasheet for recommended dilution |
Key Specifications
| Clonality | Monoclonal |
|---|---|
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate | Non-conjugated |
| Purification | Affinity chromatography |
| Clone | 5B2 |
| Catalog No. | CSB-RA286668A0HU |
| Alternative Names | Caspase-3 (CASP-3) (EC 3.4.22.56) (Apopain) (Cysteine protease CPP32) (CPP-32) (Protein Yama) (SREBP cleavage activity 1) (SCA-1) [Cleaved into: Caspase-3 subunit p17, Caspase-3 subunit p12], CASP3, CPP32 |
Immunogen / Antigen
A synthesized peptide derived from human pro Caspase 3 (Antigen species: Human)
Storage & Handling
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
This antibody has been confirmed reactive against: the species listed on the datasheet. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
A synthesized peptide derived from human pro Caspase 3
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.