CASP8 Antibody

SKU:BHA10509293
Overview
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CASP8-specific polyclonal antibody raised in Rabbit. Validated for ELISA, Western Blot, Immunohistochemistry in Human samples. Affinity-purified.
Format Unconjugated
Immunogen Recombinant
Applications 4 validated
Dilution WB:1:500-1:2000, IHC:1:10-1:100, IF:1:50
Target Aliases 43 aliases
Options selector
Catalog no. Size
CSB-PA18109A0Rb-50UG 50 ug
CSB-PA18109A0Rb-100UG 100 ug
Available Options
Size
Field Specification
Target CASP8
Antigen
  • Recombinant Human Caspase-8 protein (217-374AA)
Alternative names ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP-8; CASP8; CASP8_HUMAN; Caspase 8; Caspase 8 apoptosis related cysteine peptidase; Caspase IIX; Caspase-8 subunit p10; caspase8; CED 3; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH; MACH beta 1/2/3/4 protein; MACH5; MCH 5; MCH5; MGC78473; MORT1 associated ced 3 homolog; MORT1 associated CED3 homolog; MORT1-associated CED-3 homolog; OTTHUMP00000163717; OTTHUMP00000163720; OTTHUMP00000163724; OTTHUMP00000163725; OTTHUMP00000165062; OTTHUMP00000165063; OTTHUMP00000165064; OTTHUMP00000206552; OTTHUMP00000206582
UniProt # Q14790
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • ELISA
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
Immunogen Recombinant Human Caspase-8 protein (217-374AA)
Conjugate
  • Non-conjugated
Source This product is a polyclonal antibody purified from rabbit antiserum.
Purification Protein G purification
Form Liquid
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Storage buffer 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Catalog no. (Mfr.) CSB-PA18109A0Rb
Main SKU BHA10509293

Product Overview

Primary Antibody

This antibody targets CASP8 and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
ELISA WB:1:500-1:2000, IHC:1:10-1:100, IF:1:50-1:200
Western Blot (WB) WB:1:500-1:2000, IHC:1:10-1:100, IF:1:50-1:200
Immunohistochemistry (IHC) WB:1:500-1:2000, IHC:1:10-1:100, IF:1:50-1:200
Immunofluorescence (IF) WB:1:500-1:2000, IHC:1:10-1:100, IF:1:50-1:200
✔ Validated by CUSABIO Technology

Antibody Specifications

Target CASP8
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human
Purification Protein G Purification
Antigen / Immunogen Recombinant Human Caspase-8 protein (217-374AA)
Production This product is a polyclonal antibody purified from rabbit antiserum.
Form Liquid
Storage Buffer 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Alternative Names

ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP-8; CASP8; CASP8_HUMAN; Caspase 8; Caspase 8 apoptosis related cysteine peptidase; Caspase IIX; Caspase-8 subunit p10; caspase8; CED 3; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH; MACH beta 1/2/3/4 protein; MACH5; MCH 5; MCH5; MGC78473; MORT1 associated ced 3 homolog; MORT1 associated CED3 homolog; MORT1-associated CED-3 homolog; OTTHUMP00000163717; OTTHUMP00000163720; OTTHUMP00000163724; OTTHUMP00000163725; OTTHUMP00000165062; OTTHUMP00000165063; OTTHUMP00000165064; OTTHUMP00000206552; OTTHUMP00000206582

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetCASP8
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ConjugateUnconjugated
ReactivityHuman
PurificationProtein G Purification
Antigen/ImmunogenRecombinant Human Caspase-8 protein (217-374AA)
ProductionThis product is a polyclonal antibody purified from rabbit antiserum.
FormLiquid
Storage Buffer0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
ELISAWB:1:500-1:2000, IHC:1:10-1:100, IF:1:50-1:200
Western Blot (WB)WB:1:500-1:2000, IHC:1:10-1:100, IF:1:50-1:200
Immunohistochemistry (IHC)WB:1:500-1:2000, IHC:1:10-1:100, IF:1:50-1:200
Immunofluorescence (IF)WB:1:500-1:2000, IHC:1:10-1:100, IF:1:50-1:200

Immunogen: Recombinant Human Caspase-8 protein (217-374AA)

Antigen Species: Human

Alternative Names

ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP-8; CASP8; CASP8_HUMAN; Caspase 8; Caspase 8 apoptosis related cysteine peptidase; Caspase IIX; Caspase-8 subunit p10; caspase8; CED 3; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH; MACH beta 1/2/3/4 protein; MACH5; MCH 5; MCH5; MGC78473; MORT1 associated ced 3 homolog; MORT1 associated CED3 homolog; MORT1-associated CED-3 homolog; OTTHUMP00000163717; OTTHUMP00000163720; OTTHUMP00000163724; OTTHUMP00000163725; OTTHUMP00000165062; OTTHUMP00000165063; OTTHUMP00000165064; OTTHUMP00000206552; OTTHUMP00000206582

What species does this CASP8 antibody react with?
This antibody has been validated for reactivity against Human. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: ELISA, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
WB:1:500-1:2000, IHC:1:10-1:100, IF:1:50-1:200. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Rabbit and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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