| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Caspase-5; CASP-5; ICE(rel)-III; Protease ICH-3; Protease TY; Caspase-5 subunit p10; CASP5; ICH3; Caspase5 |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthetic peptide, corresponding to amino acids 341-386 of Human Caspase-5. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyCaspase-5 (also designated ICErel-III, TY, ICH-3 and caspase-12 in mouse), can cleave its own precursor, an activity that requires the cysteine 245 residue. Frameshift mutations in caspase-5 have been identified in MMP tumors of the endometrium, colon, and stomach, indicating the caspase-5 may be a new target gene in the microsatellite mutator pathway for cancer. The human caspase 5 gene maps to chromosome 11q22.2-q22.3 and encodes a protein whose expression is barely detectable in most tissues except brain, with highest expression levels being found in lung, liver and skeletal muscle.
Validated Applications
| Western Blot (WB) | 1:500~1:1000 |
|---|
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 10,48 kDa |
| Catalog No. | BS5645 |
| UniProt | P51878 |
| Alternative Names | Caspase-5; CASP-5; ICE(rel)-III; Protease ICH-3; Protease TY; Caspase-5 subunit p10; CASP5; ICH3; Caspase5 |
Immunogen / Antigen
Synthetic peptide, corresponding to amino acids 341-386 of Human Caspase-5.
Reactivity Notes
Caspase 5 p10 polyclonal antibody recognizes endogenous levels of total caspase-5 protein and the p10 subunit of mature caspase-5.
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB: 1:500~1:1000
This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Synthetic peptide, corresponding to amino acids 341-386 of Human Caspase-5.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
