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CC531 is a well-characterized rat adenocarcinoma cell line derived from the colon. It was originally established from a chemically induced colon tumor in a Wistar rat using 1,2-dimethylhydrazine (DMH), a potent carcinogen. The CC531 cell line is commonly utilized as a model system to study colorectal cancer mechanisms and the tumor microenvironment in vivo, particularly within the context of metastasis and immune responses. These cells are immunogenic and are often used in syngeneic rat models to investigate the efficacy of cancer immunotherapies and the interaction between cancer cells and the immune system.
In research settings, CC531 cells are employed to examine the biological processes of colorectal cancer progression, including cell proliferation, apoptosis, and metastatic behavior. The cell line has been instrumental in studying the response of colorectal cancer to various chemotherapeutic agents and radiation therapy, providing insights into the mechanisms of resistance and sensitivity to cancer treatments. Moreover, the CC531 model serves as a valuable tool for the development and optimization of novel therapeutic strategies targeting colorectal cancer, making it crucial for translational cancer research.
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Tumorigenic: Yes, in nude mice, syngeneic WAG-Rij rats
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% FBS, 20 mM HEPES
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 1 to 2 x 104 cells/cm2 will result in a confluent monolayer within 3 to 4 days.
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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