CCD-1095Sk cell

SKU:BHC11101081
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Overview
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CCD-1095Sk cell is a cell line (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Fibroblast. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Disease model Ductal carcinoma
Morphology Fibroblast
Growth Properties Adherent
Tissue Skin
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Catalog no. Size
300642 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 300642
Species Human
CCD-1095Sk is a fibroblast cell line derived from the skin of a human male. It was established from a biopsy of uninvolved skin taken from a patient who had a squamous cell carcinoma. This cell line is utilized primarily in studies that explore the interactions between skin cells and cancerous cells, particularly how non-cancerous cells in the tumor microenvironment can influence tumor growth and progression. The CCD-1095Sk cell line is therefore valuable for cancer research, specifically for understanding the stromal aspects of skin cancer. The CCD-1095Sk cells exhibit a fibroblast morphology, characterized by a spindle-shaped, elongated form typical of connective tissue cells that produce extracellular matrix components essential for tissue repair and structural integrity. These cells are adherent, grow in monolayers, and are known for their robustness in various in vitro experimental conditions. They are used to model fibroblast behavior in normal skin and to examine changes in fibroblast activity under cancerous conditions, which can include the secretion of growth factors, cytokines, and matrix metalloproteinases. As such, they provide an invaluable tool for pharmacological studies and the development of therapeutic strategies targeting the tumor environment.

SKU:BHC11101081

  • cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
  • supplements: Supplement the medium with 10% FBS and 1% NEAA
  • dissociationReagent: Accutase
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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