CCD-18Lu cell

SKU:BHC11101465
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Overview
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CCD-18Lu cell is a Fibroblast cell line derived from African American (Female). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Fibroblast. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Morphology Fibroblast
Growth Properties Adherent
Tissue Lung
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Catalog no. Size
305248 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305248
Species Human
The CCD-18Lu cell line is derived from normal lung fibroblasts of a human adult. These cells were established from the lung tissue of a male patient and are commonly used as a model for studying the behavior of normal human lung fibroblasts. The CCD-18Lu cell line exhibits typical fibroblast morphology, characterized by spindle-shaped cells that grow adherently in culture and form a monolayer. Researchers utilize CCD-18Lu cells in various studies related to pulmonary biology, including investigations into lung development, repair, and fibrosis. These cells are instrumental in understanding the mechanisms underlying normal lung function and the response of lung fibroblasts to different environmental stimuli, such as cytokines, growth factors, and extracellular matrix components. Additionally, CCD-18Lu cells are employed in studies examining the effects of various drugs and compounds on lung fibroblast proliferation, differentiation, and collagen production. In cancer research, CCD-18Lu cells serve as a control or reference cell line to compare with lung cancer cell lines, helping to identify specific molecular and cellular alterations associated with lung cancer progression. By providing insights into the behavior of normal lung fibroblasts, the CCD-18Lu cell line contributes to the development of therapeutic strategies for treating lung diseases, including fibrosis and cancer.

SKU:BHC11101465

  • cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
  • supplements: Supplement the medium with 10% FBS and 1% NEAA
  • dissociationReagent: Accutase
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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