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The CCF-STTG1 cell line is a human astrocytoma cell line derived from a cerebral tumor. This cell line is of particular interest in cancer research due to its origin from a malignant astrocytoma, which is a type of brain tumor derived from astrocytes cells that support nerve cells. The CCF-STTG1 cells exhibit a robust capacity for proliferation and maintain several characteristics typical of astrocytes, making them a valuable model for studying the biological and molecular mechanisms of tumorigenesis in the central nervous system.
CCF-STTG1 cells are used extensively in oncological studies, particularly in those examining the genetic and epigenetic alterations that contribute to brain tumor pathology. These cells are useful in assays for drug screening and resistance, gene expression analysis, and for studying the effects of cancer therapeutics on cell viability, proliferation, and apoptosis. Researchers also utilize this cell line to explore the complex signaling pathways involved in cancer progression and to test novel therapeutic targets for glioblastoma and other astrocytomas.
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Antigen expression: HLA DR (on approx. 25% of cells)
- cultureMedium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
- supplements: Supplement the medium with 10% FBS
- dissociationReagent: Accutase
- subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
- seedingDensity: 2 x 104 cells/cm2 will result in a confluent monolayer within 4 days.
- fluidRenewal: 2 to 3 times per week
- postThawRecovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
- freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
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