| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, and ILA |
| Formulation | |
| Molecular Weight | |
| Product Type | |
| Shipping | |
| Species | |
| Storage | |
| UniProt # |
Scientific Background
Mouse secreted CD137, also known as Tumor Necrosis Factor Receptor Superfamily member 9, TNFRSF9, and ILA, GenBank Accession No. NM_011612, a.a. 24-187 fused at the C-terminus to the Fc portion of human IgG1, expressed in a HEK293 cell expression system. MW = 44 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Product Description
Insect Cells (Sf9) ExpressionRecombinant CD137 Mouse protein is produced using a validated Insect Cells (Sf9) expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | P20334 |
|---|---|
| Expression System | Insect Cells (Sf9) |
| Amino Acids / Region | 24-187 |
| Affinity Tag | Fc fusion (hIgG1) |
| Molecular Weight | 44 kDa |
| Formulation | 8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol. |
| Storage | At least 6 months at -80°C. |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles. Storing diluted protein is not recommended, if necessary, include carrier protein (BSA 0.1 - 0.5%).
This protein was produced in Insect Cells (Sf9). Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
This protein spans amino acids 24-187. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Useful for studying protein binding and screening small molecular inhibitors Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
At least 6 months at -80°C. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
- Wilcox, R.A., et al., J. Clin. Invest. 2002; 109(5): 651-659.
- Foell, J. et al., J. Clin. Invest. 2003; 111(10): 1505-1518.
