| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | C163A_HUMAN; CD 163; CD163; CD163 antigen; CD163 molecule; Hemoglobin scavenger receptor; M130; M130 antigen precursor; Macrophage associated antigen; MM130; OTTHUMP00000238617; OTTHUMP00000238618; OTTHUMP00000238619; OTTHUMP00000238620; SCARI1; Scavenger receptor cysteine rich type 1 protein M130; sCD163; Soluble CD163 |
| Clonality | |
| Conjugate | |
| Form | Liquid |
| Host | |
| Immunogen | Recombinant Human Scavenger receptor cysteine-rich type 1 protein M130 protein (801-940AA) |
| Isotype | |
| Product Type | |
| Reactivity | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Storage | |
| Target | |
| UniProt # |
Overview
This is a polyclonal anti-CD163 antibody raised in Rabbit, conjugated with Biotin, with confirmed utility in ELISA. It is designed to detect CD163 protein in Human and supports researchers working in immunology contexts.
Key elements and design rationale
- Immunogen: Recombinant Human Scavenger receptor cysteine-rich type 1 protein M130 protein (801-940AA) — determines the epitope region; confirm compatibility with sample preparation and expected post-translational modifications.
- Host species (Rabbit): Requires anti-rabbit-IgG secondary reagents for indirect detection.
- Polyclonal format: Recognizes multiple epitopes for robust signal; inherent lot-to-lot variability requires appropriate controls.
- Isotype (IgG): Matched secondary antibodies and isotype controls required. Compatible with standard Protein A/G purification workflows.
- Purification (Protein G purification): Enriches for specific immunoglobulin classes; reduces non-specific background vs. crude antisera.
- Biotin conjugate: Amplified detection via streptavidin/avidin reporters. Block endogenous biotin in biotin-rich tissue samples.
Biological background
CD163 (also referred to as C163A_HUMAN, CD 163, CD163, CD163 antigen, CD163 molecule, Hemoglobin scavenger receptor, M130, M130 antigen precursor, Macrophage associated antigen, MM130, OTTHUMP00000238617, OTTHUMP00000238618, OTTHUMP00000238619, OTTHUMP00000238620, SCARI1, Scavenger receptor cysteine rich type 1 protein M130, sCD163, Soluble CD163) is a protein target studied in Human systems. Expression, subcellular localization, and post-translational modifications vary across cell types and disease states — factors critical to antibody-based detection design. Consult UniProt, NCBI Gene, and primary literature for current annotation of CD163 biology in immunology.
Common research applications
- ELISA: Quantification of soluble target in biological fluids or culture supernatants. Ensure samples are within the linear detection range.
Notes for experimental interpretation
- Isotype controls: Use an isotype-matched (IgG from Rabbit) control at equivalent concentration to assess non-specific background.
- Cross-reactivity: Polyclonal preparations may cross-react with related proteins. Orthogonal validation (siRNA, KO lysate) is recommended.
- Matrix effects: Sample matrix can affect performance; pilot dilution linearity and spike-recovery experiments are advised for quantitative studies.
- Species reactivity: Confirmed for Human. Extrapolation to untested species requires empirical validation.
This product is a polyclonal antibody purified from rabbit antiserum.
CD163 is a protein target in Human biology. This polyclonal antibody raised in Rabbit is designed to detect CD163 in ELISA applications, with IgG isotype.
Reported reactive against Human. Immunogen derived from Human. Cross-reactivity with other species should not be assumed without documented data or empirical testing.
Reported for ELISA. Each format requires independent dilution optimization and appropriate controls. Unlisted applications require empirical testing.
Biotin enables streptavidin/avidin-amplified detection. Block endogenous biotin in biotin-rich tissues.
(1) Isotype control — IgG from Rabbit at matching concentration; (2) Positive control — known CD163-expressing cell/tissue; (3) Negative control — knockdown/knockout sample for specificity; (4) Dilution linearity — verify proportional signal decrease in your matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
