| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A peptide sequence specific to CD3 zeta protein was used as the immunogen for the CD247 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
CD247 Antibody / CD3 zeta is a anti-CD247 Rabbit antibody Recombinant Rabbit Monoclonal clone IHI-3 supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Human. Reported localization: Cell membrane.
Key elements and design rationale
- Target: CD247
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone IHI-3, isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IHC-P
Biological background
CD247 is predominantly expressed in T cells and natural killer (NK) cells, where it regulates immune responses against pathogens and abnormal cells. Alterations in CD247 expression or function have been linked to immunodeficiencies, autoimmune diseases, and cancer, making it a valuable focus for immunological research.
Using a high-quality CD247 antibody enables precise detection in applications such as western blot, flow cytometry, and immunohistochemistry. A CD247 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
