CD249 Polyclonal Antibody

SKU:BHA12103768
Overview
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ENPEP-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, Immunohistochemistry, ELISA in Human, Mouse, Rat samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen Synthetic Peptide
Molecular Weight ~109.2 kDa
UniProt Q07075
Gene ID Human: 2028; Mouse: 13809; Rat: 64017
Applications 3 validated
Options selector
Catalog no. Size
BT-AP01429-20UL 20 uL
BT-AP01429-50UL 50 uL
BT-AP01429-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-AP01429
Alternative Names ENPEP; Glutamyl aminopeptidase; EAP; Aminopeptidase A; AP-A; Differentiation antigen gp160; CD249
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID Human: 2028; Mouse: 13809; Rat: 64017
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from the Internal region of human ENPEP. AA range:671-720
Isotype
  • IgG
Molecular Weight ~109.2 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
  • Rat
Storage -20°C for 1 year
Target ENPEP

Product Overview

Primary Antibody

This antibody targets ENPEP and detects endogenous levels of ENPEP protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target ENPEP
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human, Mouse, Rat
Purification Affinity Purification
Molecular Weight ~109.2 kDa
Concentration 1 mg/ml
Immunogen The antiserum was produced against synthesized peptide derived from the Internal region of human ENPEP. AA range:671-720
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 2028; Mouse: 13809; Rat: 64017
UniProt Q07075 (Human: Q07075; Mouse: P16406; Rat: P50123)

Scientific Background

ENPEP (Glutamyl Aminopeptidase) is a Protein Coding gene. Diseases associated with ENPEP include neurogenic hypertension and gestational choriocarcinoma. Among its related pathways are Agents Acting on the Renin-Angiotensin System Pathway, Pharmacodynamics and Peptide hormone metabolism. GO annotations related to this gene include peptidase activity and aminopeptidase activity. An important paralog of this gene is ERAP2.

Alternative Names

ENPEP; Glutamyl aminopeptidase; EAP; Aminopeptidase A; AP-A; Differentiation antigen gp160; CD249

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetENPEP
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman, Mouse, Rat
PurificationAffinity Purification
Molecular Weight~109.2 kDa
Concentration1 mg/ml
ImmunogenThe antiserum was produced against synthesized peptide derived from the Internal region of human ENPEP. AA range:671-720
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 2028; Mouse: 13809; Rat: 64017
UniProtQ07075 (Human: Q07075; Mouse: P16406; Rat: P50123)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

ENPEP (Glutamyl Aminopeptidase) is a Protein Coding gene. Diseases associated with ENPEP include neurogenic hypertension and gestational choriocarcinoma. Among its related pathways are Agents Acting on the Renin-Angiotensin System Pathway, Pharmacodynamics and Peptide hormone metabolism. GO annotations related to this gene include peptidase activity and aminopeptidase activity. An important paralog of this gene is ERAP2.

Alternative Names

ENPEP; Glutamyl aminopeptidase; EAP; Aminopeptidase A; AP-A; Differentiation antigen gp160; CD249

What species does this antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), Immunohistochemistry (IHC), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of ENPEP?
The predicted molecular weight of ENPEP is ~109.2 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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