| Field | Specification |
|---|---|
| Target | |
| Alternative names | Programmed cell death 1 ligand 1 (PD-L1) (PDCD1 ligand 1) (Programmed death ligand 1) (B7 homolog 1) (B7-H1) (CD antigen CD274) |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide from human protein at AA range: 181-230 |
| Conjugate | |
| Concentration | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
This gene encodes an immune inhibitory receptor ligand that is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015],
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes CD274. It is validated for Immunohistochemistry, Immunofluorescence, ELISA.
Validated Applications
| Applications | Immunohistochemistry, Immunofluorescence, ELISA |
|---|---|
| IHC-p 1 | 50-200, ELISA 1:10000-20000 IF 1:100-300 Not yet tested in other applications. |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthetic peptide from human protein at AA range: 181-230
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Immunogen | Synthetic peptide from human protein at AA range: 181-230 |
| Concentration | 1 mg/mL |
| Subcellular Location | Cell membrane ; Single-pass type I membrane protein . Early endosome membrane ; Single-pass type I membrane protein . Recycling endosome membrane ; Single-pass type I membrane protein . Associates with CMTM6 at recycling endosomes, where it is protected from being targeted for lysosomal degradation. .; [Isoform 1]: Cell membrane ; Single-pass type I membrane protein .; [Isoform 2]: Endomembrane system ; Single-pass type I membrane protein . |
| Swiss-Prot | Q9NZQ7 |
| Gene ID | 29126 |
| Storage | -20°C/1 year |
Reactivity Notes
Validated reactivity: Human, Rat, Mouse. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for CD274 research from ELK Biotechnology, or contact us for alternative formats.
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